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conferenceseries
.com
Volume 5, Issue 3 (Suppl)
J Infect Dis Ther, an open access journal
ISSN:2332-0877
Infectious Diseases 2017
August 21-23, 2017
3
rd
Annual Congress on
Infectious Diseases
August 21-23, 2017 San Francisco, USA
Pavel G Alexyuk et al., J Infect Dis Ther 2017, 5:3 (Suppl)
DOI: 10.4172/2332-0877-C1-027
Influence of the structure of viral antigens on the activity of adaptive immunity
Pavel G Alexyuk, Madina S Alexyuk, Aizha S Turmagambetova, Elmira S Omirtaeva, Irina A Zaitseva, Andrey P Bogoyavlenskiy
and
Vladimir E Berezin
Institute of Microbiology and Virology, Kazakhstan
M
ost modern vaccines are minimal compositions which possess low immunogenicity, while large aggregated antigen
particles are required to activate a long-term immune response. Therefore, the study of molecular basis of the
interaction of antigens with immunocompetent cells and the mechanisms of the immune response remains an important
problem of vaccinal prevention. In this case, are demand to develop of new delivery systems and antigen presentation that
can enhance immunogenicity and effectiveness of vaccine preparations without use of additional components. The purpose
of this research was to identify the correlation between the form of supramolecular organization of viral antigens and the
induction of inflammatory reactions in the organism. Mice weighing 20-25 grams were immunized once intraperitoneally
with purified glycoprotein antigens (HA+NA) obtained from the influenza virus, strain A/Aichi/2/68 (H3N2), having the
following forms of supramolecular organization: Micelles; immunostimulating nanocomplexes (virus-like nanostructures 60
- 80 nm in diameter formed by antigens, lipids and plant saponins (Quil-A and SO)); nanostructured chitosan with adsorbed
antigens. For comparison, animals were also immunized with purified viral particles. Day later, peritoneal exudate cells were
collected for isolation of nucleic acids. Expression level of genes responsible for the synthesis of IgA, IgG2a antibodies was
determined. A highest gene expression activity was detected after mice immunization with immunostimulating complexes
based on viral antigens, saponins (Quil A; SO) and lipids. Sufficiently high expression level of studied immunoglobulins,
but less than immunostimulating complexes, was recorded by immunization of mice with intact virus particles and chitosan
nanostructures. Viral glycoprotein antigens in the form of classical micelles showed the lowest activity in stimulation of
studied genes. Thus, it was shown that the expression level of genes responsible for the induction of IgA and IgG2a antibodies
at immunization of experimental animals with different structural forms of viral antigens was largely depend on molecular
structure of viral antigen.
Biography
Pavel GAlexyuk has her expertise in evaluation and passion in improving the health and wellbeing. Her open and contextual evaluation model based on responsive
constructivists creates new pathways for improving healthcare. She has built this model after years of experience in research, evaluation, teaching and administration
both in hospital and education institutions. The foundation is based on fourth generation evaluation (Guba & Lincoln, 1989) which is a methodology that utilizes
the previous generations of evaluation: measurement, description and judgment. It allows for value-pluralism. This approach is responsive to all stakeholders and
has a different way of focusing.
pagenal@bk.ru