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conferenceseries
.com
Volume 5, Issue 3 (Suppl)
J Infect Dis Ther, an open access journal
ISSN:2332-0877
Infectious Diseases 2017
August 21-23, 2017
3
rd
Annual Congress on
Infectious Diseases
August 21-23, 2017 San Francisco, USA
M. Celeste Nicolao et al., J Infect Dis Ther 2017, 5:3 (Suppl)
DOI: 10.4172/2332-0877-C1-027
Exosome-like vesicles secreted by Echinococcus granulosus larval stage contain proteins involved
in parasite-host communication
M. Celeste Nicolao, Christian Rodriguez Rodrigues
and
Andrea C. Cumino,
Mar del Plata National University, Exact and Natural Science Faculty, Argentina
S
tatement of the Problem: Human echinococcosis is a zoonotic cestode disease caused by Echinococcus sp. larval stage. These
helminth parasites lack digestive and excretory system but they have developed active endocytic-exocytic cellular processes
to regulate metabolite uptake and excretion. The purpose of this study is to analyze the cestode exosome-like production
and to characterize these vesicles focusing on the parasite-host interaction. Methodology & Theoretical Orientation: Viable
protoscoleces and metacestodes were in vitro cultured in presence of loperamide or in control conditions and viability and
calciumconcentrationweredetermined. Additionally, extracellular vesicleswerepurified fromparasite-culturemediumthrough
several centrifugation and ultracentrifugation steps and were analyzed by confocal imaging, TEM, western blot and proteomic
analysis. Findings: Loperamide reduced the viability of both larval stages in a dose-dependent manner, provoked a cytosolic
calcium level increment and induced a higher density of vesicles respect to the control. In addition, TEM analysis enabled the
vesicles morphological characterization and the identification of abundant exosomes (30-100 nm vesicles with cup-shaped
morphology). Finally, a large amount of exosomal proteins have been identified by proteomic analysis, among them Alix and
TSG101 which are components of the endosomal sorting complex required for transport and are considered exosomal protein
markers; Syntenin-1 implicated in the regulation of exosome biogenesis; Tetraspanins, related to cell adhesion (in particular
CD9, whose expression was corroborated by WB); proteins involved in vesicle related transport (such as rab proteins, syntaxin-
binding protein) and proteins involved in host immune response, parasite antigens and uncharacterized proteins which are of
special interest for their putative role in parasite-host interaction. Conclusion & Significance: Echinococcus granulosus secretes
exosome-like vesicles which could be involved in the host immune response. Further studies are needed to fully investigate
these vesicles which could be involved in parasite establishment and immune tolerance that guarantees cestode survival.
Biography
M. Celeste Nicolao has a PhD in Biological Science (National University of Mar del Plata, 2016) and she is currently working as a postdoc under the direction of
Prof. Dr. Andrea Cumino. She has been serving as assistant teacher for subjects such as Introduction to Biology, Clinic Microbiology and Basic Immunology that are
part of the Biology and Biochemistry courses of study. She participates in several research projects on Parasitology and has published original articles. Currently,
her research is focused on the study of molecular and biochemical mechanisms involved in Echinococcus sp- host interaction.
celestenicolao@hotmail.com