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Volume 6
Journal of Infectious Diseases & Therapy
ISSN: 2332-0877
Infection Prevention 2018
December 06-07, 2018
December 06-07, 2018 | Valencia, Spain
14
th
World Congress on
Infection Prevention and Control
J Infect Dis Ther 2018, Volume 6
DOI: 10.4172/2332-0877-C6-054
Detectionof themcr-1 colistinresistance gene andextended-
spectrumbeta-lactamase (esbl)-producing
Escherichia coli
from
poultry in Qatar
Nahla Eltai
Qatar University, Qatar
A
ntimicrobial resistance (AMR) is a growing public health concern worldwide and is one of the top health challenges
facing humanity in the 21st century. AMR among Enterobacteriaceae is rapidly increasing especially to third-generation
cephalosporins and carbapenems. Further, strains carrying mobilized colistin resistance (mcr) genes 1 & 2 have been isolated
from humans, food-producing animals, and environment. Uncontrolled use of antibiotics in animals in large scale could
be one of the major contributing factors to generation and spread of antibiotic resistance. No studies have been done to
evaluate antimicrobial resistance in animals in Qatar. This study aimed at establishing a primary baseline data for prevalence
of antimicrobial resistance among food animals in Qatar. 172 fecal samples were obtained from two broiler farms and one live
bird market in Qatar and 90 Escherichia coli (E. coli) bacteria were isolated and subjected to antimicrobial susceptibility testing
using E test method. 90% (81/90) of the isolates were resistant to at least one of the 16 tested antibiotics. 15.5% (14/90) of the
isolates were colistin resistant, 2.2% (2/90) were extended spectrum β lactamase (ESBL) producers and similar percentage were
multi-drug resistant (MDR) to four antibiotic classes. ESBL-producing E. coli and colistin resistant isolates were confirmed
using double disc susceptibility testing and PCR, respectively. In summary, our results indicate high antimicrobial resistance
in food producing animals in Qatar, including ESBL and colistin resistance. Such AMR bacteria could be easily transmitted
to humans through consumption of undercooked food or noncompliance with hygiene practices, which mandates prompt
development and implementation of stewardship program to control andmonitor the use of antimicrobial agents in community
and agriculture.
nahla.eltai@qu.edu.qa