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.com
Volume 6
Journal of Infectious Diseases & Therapy
ISSN: 2332-0877
Infection Prevention 2018
December 06-07, 2018
December 06-07, 2018 | Valencia, Spain
14
th
World Congress on
Infection Prevention and Control
Phenotypic and genotypic determination of nasal staphylococcus aureus and mrsa carriage in trainee
students of health services vocational school
Cemil Demir
1
, Akin Yi
ğ
in
2
Mehmet Demirci
3
and
Serap KılıçAltun
4
1
Vocational School of Health Services, Department of the Medical Documentation and Secretarial, Mardin Artuklu University, Mardin, Turkey
2
Harran University Veterinary Faculty, Department of Genetics Şanlıurfa, Turkey
3
Beykent University, Medical Faculty, Department of Medical Microbiology Istanbul, Turkey
4
Harran University Veterinary Faculty, Department of Food Hygiene Şanlıurfa, Turkey
Background:
Methicillin-resistant Staphylococcus aureus (MRSA) are an important bacterial pathogen, resistant to beta-lactam
antibiotics and are frequently isolated severe infection in hospital settings. It has been reported that long-term hospitalized
individuals, such as health workers and medical students with high chances of carrying these strains, may be potential sources
of nosocomial infections.
Objectives:
In our study, it is aimed to investigate epidemiologically phenotypic and genotypic state of carriage which occurs
before and after the laboratory internship. In addition, the difference between phenotypic and genotypic methods will be
examined.
Material and Methods:
Nasal swab samples collected from 180 trainee students before and after the laboratory internship
period at Medical Laboratory Department without any health problems between in 2014 and 2016. Phenotypically for
conventinal methods and genotypically for real-time PCR were used to detect S.aureus and MRSA.
Conclusions:
Nasal S.aureus carriage was found 12 (6,66%) and 21 (11,66%) of the samples taken before and after the internship
period respectively. Nasal MRSA carriage was found 3 (1,66%) and 5 (2,77%) of the samples taken before and after the internship
respectively. During the 14-week internship period (one day per week), both S.aureus and MRSA carriers amount increased
in trainees. All phenotypic results also confirmed by real-time PCR. As a result, our results suggested that colonization of this
bacteria in the hospital environment should be improved and caution should be taken in terms of nosocomial infections.
cemildemir@ymail.comJ Infect Dis Ther 2018, Volume 6
DOI: 10.4172/2332-0877-C6-054