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Journal of Infectious Diseases & Therapy |ISSN: 2332-0877 | Volume 6
June 25-26, 2018 | Vancouver, Canada
3
rd
International Conference on
2
nd
International Conference on
Infection, Disease Control and Prevention
Microbial Pathogenesis & Infectious Diseases
&
Frequency of
PmrA
,
PmrB
and
mcr-1
genes in
pseudomonas aeruginosa
isolates from cystic fibrosis
patients of mofid children hospital, Tehran, Iran
Mojdeh Hakemi Vala
Islamic Azad University, Iran
Introduction:
The emergence of multidrug- resistant (MDR) in gram negative pathogens such as
P. aeruginosa
has become an
important challenge to worldwide public health, recently (1). Colistin as a last line of treatment against MDR gram negative
bacteria has been propounded (2). Recent studies have been demonstrated that colistin resistance is adaptive and regulated via
PmrA
and
PmrB
as a two component regulatory system (3). The plasmid borne colistin resistance gene
mcr-1
was primarily
recognized from China (4). Due to the raising of antibiotic resistance, in this study prevalence of
mcr-1
gene and the mutation
in
pmrA
and
pmrB
genes in
P. aeruginosa
isolates from sputum of Cystic Fibrosis (CF) patients was investigated.
Methods:
41 isolates of
P. aeruginosa
from sputum of CF patients in Mofid Children Hospital during Apr-Sep 2017 were
collected. According to CLSI guideline 2017, antibiotic susceptibility test (AST) was applied by using the disk diffusion method.
Detection of
pmrA
,
pmrB
and
mcr-1
genes was performed by PCR and further sequencing was administrated for finding the
mutations.
Results:
Among 41 isolates of
P. aeruginosa
22 (53.65%) were resistant to Amikacin, 21 (51.21%) to Ofloxacin and Cefepime, 20
(48.78%) to Imipenem, 19 (46.34%) to Ceftazidime and Ciprofloxacin, 18 (43.9%) to Aztreonam, 17 (41.46%) to Piperacillin,
16 (39.02%) to Gentamicin, 14 (34.14%) to Pipracillin-Tazobactam and 1 (2.43%) to Colistin. PCR results showed that all of
the isolates had
pmrA
and
pmrB
genes and all of them were negative for
mcr-1
. One of the isolates show some mutations in
pmrB
gene.
Conclusion:
The results of this study showed that colistin is the best choice for treatment. Using the molecular tests is necessary
and have a determinative role to prescription of antibiotics by physicians.
Biography
Mojdeh Hakemi Vala is working at Departmentof Microbiology, Medical school, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
mojdeh_hakemi@yahoo.comMojdeh Hakemi Vala, J Infect Dis Ther 2018, Volume 6
DOI: 10.4172/2332-0877-C2-041