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Industrial Microbiology 2016
October 17-18, 2016
Volume 8, Issue 5(Suppl) J Microb Biochem Technol
ISSN:1948-5948 JMBT, an open access journal
conferenceseries .com
October 17-18, 2016 Kuala Lumpur, Malaysia
Industrial & Pharmaceutical MicrobiologyInternational Conference and Summit on
Hemanth Nag Noothalapati Venkata, J Microb Biochem Technol 2016, 8:5(Suppl)
Imaging yeast cell wall architecture and studying the effect of antifungal drug Terbinafine at the
single cell level by Raman microscopy
Hemanth Nag Noothalapati Venkata
Shimane University, Japan
Fungal cell wall is a dynamic organelle that plays a vital role, particularly in cellular growth, elongation and division. Any
modification or disruption of the wall leads to lysis and cell death, hence serving an excellent target for anti-fungal drugs.
Chemically, the cell wall architecture in fungi is complex comprising mainly polysaccharides (glucan, mannan and chitin) and
a small proportion of glycoproteins. Traditionally, electron microscopy and biochemical extraction methods were employed
while recently immunocytochemical analysis is used to understand its structure.The former lacks chemical specificity requiring
genetically modified cells to study different structures in detail while the later involves development of fluorescent monoclonal
antibodies specific to glycosidic linkages among cell wall polysaccharides. Hence we aim to develop a label-free method based
on confocal Raman microscopy to visualize distribution of various polysaccharide components of fungal cell and spore wall.
Fission yeast Schizosaccharomyces pombe is used as a model to demonstrate our method. First, space-resolved Raman spectra
from lipid droplets, cytoplasm and cell wall were obtained to identify marker bands for individual components followed
by imaging. Then, by employing multivariate curve resolution (MCR) analysis, we successfully separated Raman spectra of
several pure bio-macromolecular components. We then studied pharmacokinetics and pharmacodynamics of antifungal drug
terbinafine at the single cell level in yeast model. We believe that our method will help in understanding the complex fungal
spore wall architecture and eventually lead to advancements in drug discovery and development in the future.
Biography
Hemanth Nag Noothalapati Venkata has completed his PhD from National Chiao Tung University, Taiwan. During his PhD, he studied spatio-temporal relationship
between proteome and lipid droplet in single fission yeast cells in vivo by Raman microscopy. He then developed methods to study single cell biochemistry utilizing
carbon isotopes during his Post doctorate at Ultimate Spectroscopy and Imaging Laboratory, NCTU. Later he moved to Shimane University, Japan as an Assistant
Professor and has been actively working on medical and biological applications of Raman microspectroscopy.