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Journal of Analytical & Bioanalytical Techniques | ISSN: 2155-9872 | Volume 9
World HPLC, Separation Techniques & Pharmacovigilance
World Analytical Chemistry & Mass Spectrometry
18
th
International Conference on
August 29-30, 2018 | Toronto, Canada
&
A portable chip for microextraction and subsequent optical detection in a single step: Determination
of norfloxacine in water samples
Maria Ramos Payan
1
and
Andreu Llobera
2
1University of Seville, Spain
2Technology & Innovation, Germany
I
n recent years, the miniaturization of new methodologies has become a dominant trend due to the advantages they present.
On the one hand, microextraction techniques integrated into microfluidic devices on the chip have been able to be connected
online to instrumental techniques for direct analysis. The development of new detection devices using optical fibers allows
measuring new compounds more quickly and requiring less sample volume.
In this work, we present for the first time the coupling between two miniaturized systems: a microextraction microchip
device bases liquid phase microextraction and an optical detection device using optical fibers (fluorescence), allowing on-line
determination in a single portable device using a very low sample volume. The first step was a clean-up sample treatment and
the extraction of the analyte (norfloxacin), whereas the second step was the direct determination of norfloxacin using optical
fibers. The microextraction procedure was carried out at optimal extraction conditions (dihexilether as an organic solvent, pH
3 as the sample solution, pH 12 as acceptor solution and 1 µL/min as flow rate for sample and acceptor solution). Finally, the
device has been successfully applied to environmental samples.
Biography
Maria Ramos Payan has expertise in improving sample preparation techniques focused on microfluidic-chip devices as miniaturization. The novelty of her microfluidic
devices offers more advantages than the existing methodologies. Maria has worked at different institutions (the University of Seville, University of Huelva, University
of Lund, University of Copenhagen, University of North Carolina, USA, Microelectronic National Center of Barcelona and Universitat Autónoma de Barcelona).
Currently, she works at the University of Seville with the aim of implementing optical detection into microfluidic devices for multiple different applications.
ramospayan@us.esMaria Ramos Payan et al., J Anal Bioanal Tech 2018, Volume 9
DOI: 10.4172/2155-9872-C1-027