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Journal of Analytical & Bioanalytical Techniques | ISSN: 2155-9872 | Volume 9
World HPLC, Separation Techniques & Pharmacovigilance
World Analytical Chemistry & Mass Spectrometry
18th International Conference on
August 29-30, 2018 | Toronto, Canada
&
Advances in determination of Alzheimer’s β-amyloid peptide
Alzheimer’s disease (AD), themost common type of dementia in the elderly, is aprogressive anddevastatingneurodegenerative
disease causing memory loss, impaired thinking and other symptoms. β-amyloid peptide (Aβ) indicates a biomarker for
an AD in cerebrospinal fluid, blood, plasma and serum. Therefore, it is important to determine the Aβ concentration for early
diagnosis and treatment of AD. In this study, micellar electrokinetic capillary chromatography (MEKC) combined with an on-
line preconcentration method sweeping was established to determine Aβ1-42. Electrophoresis was carried out at a constant
voltage of 5 kV in an uncoated fused-silica capillary. The 15 mmol/L borate buffer containing 20 mmol/L sodium dodecyl
sulfate (SDS) with pH 9.3 was used as the running buffer. The samples were injected into the capillary by applying a pressure
of 50 mbar for 60s. Under the optimal conditions, the detection limit of Aβ1-42 was as low as 0.08 μmol/L. The recoveries of
the normal addition method in real human serum sample analysis were found to be between 89.2% and 98.5%. The relative
standard deviation (RSD) of the determination was less than 6%.
Biography
Maotian Xu has completed his PhD at the age of 42 years from Northwest University and postdoctoral studies from Central South University. He is the director of
Henan Key Laboratory of Biomolecular Recognition and Sensing. He has published more than 40 papers in reputed journals.
Yintang Zhang has completed his PhD at the age of 29 years from Central South University. He is the director of Henan Joint International Research Laboratory of
Chemo/Biosensing and Early Diagnosis of Major Diseases. He has published more than 25 papers in reputed journals.
Maotian Xu & Yintang Zhang
Shangqiu Normal University, China
Maotian Xu et al., J Anal Bioanal Tech 2018, Volume 9
DOI: 10.4172/2155-9872-C1-027