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Journal of Analytical & Bioanalytical Techniques | ISSN: 2155-9872 | Volume 9
World HPLC, Separation Techniques & Pharmacovigilance
World Analytical Chemistry & Mass Spectrometry
18
th
International Conference on
August 29-30, 2018 | Toronto, Canada
&
Fabric Phase Sorptive Extraction (FPSE):Anovel strategy in metabolomics sample preparation for disease
biomarker discovery
M
etabolomics plays an important role in discovering potential disease biomarkers from blood plasma or serum samples.
Due to the distinctive complexity of whole blood as the sample matrix, either plasma or serum are used as the primary
sample in metabolomics biomarker discovery research. During the transformation of whole blood into plasma or serum
followed by extraction of targeted or non-targeted metabolites using conventional sample preparation techniques including
solid phase extraction (SPE) and liquid-liquid extraction (LLE), a significant portion of the analytical information disappears,
resulting in negligible success in discovering potential disease biomarkers. Fabric phase sorptive extraction (FPSE), a new
generation sample preparation technology, has offered a paradigm shift approach in metabolomics sample preparation. FPSE
innovatively combines the benefits of solid phase extraction (SPE) (works under exhaustive extraction principle) and solid phase
microextraction (works under equilibrium extraction principle) into a single sample preparation technology platform. FPSE
utilizes a flexible and permeable fabric substrate, coated with high-performance sol-gel sorbents as the extraction media. This
uniquely designed extraction medium is capable of extracting target analyte(s) directly from whole blood. Due to the special
geometry of FPSE medium (flexible, flat and permeable) and sponge-like porous architecture of sol-gel sorbents, rapid analyte
mass transfer occurs between the bulk sample and the extraction medium, resulting in a near-exhaustive extraction within
a fraction of time required for other comparable sample preparation techniques. FPSE is particularly suitable for analyzing
target analytes e.g., metabolites, biomarkers directly from whole blood without requiring any protein precipitation or other
pre-extraction sample cleaning/manipulation. After extracting the target analyte(s) directly from the whole blood sample, FPSE
media is exposed to a small volume of organic/organo-aqueous solvent for eluting the extracted analyte(s). The low viscosity
of the organic solvent, the capillary force of the fabric support and sponge-like porous sol-gel network allows fast diffusion of
organic solvent into the FPSE medium for quick and complete recovery of the extracted analyte(s). As a result, FPSE completely
eliminates time-consuming and error-prone solvent evaporation and sample reconstitution step often considered as an integral
part of solid phase extraction/liquid-liquid e work-flow. During the solvent-mediated elution/back-extraction, any protein or
matrix interferents adhered to the FPSE medium precipitates out and a final centrifugation of the resulting solution prior to
injecting into the analytical instrument ensures clean particle-free highly concentrated target analyte(s). Fabric phase sorptive
extraction has already developed a large number of sol-gel sorbents specifically suitable for polar metabolites/biomarkers such
as sol-gel polyethylene glycol, sol-gel chitosan, sol-gel Carbowax 20M, sol-gel polycaprolactone-dimethylsiloxane-caprolactone
to name a few. These high-efficiency sorbents have been found equally effective for analytes with a wide range of polarity. As a
consequence, searching for a new disease biomarker from whole blood in presence of numerous endogenous and exogenous
interferents is no longer a wishful thinking but an achievable reality. In the current talk, some new and fascinating data on
metabolomics sample preparation using FPSE and a comparison between FPSE and conventional sample preparation techniques
will be presented.
Biography
Abuzar Kabir, a Research Assistant Professor at the International Forensic Research Institute (IFRI), Department of Chemistry and Biochemistry, Florida International
University (FIU), Miami, Florida, USA, is a Separation Scientist and Materials Chemist. He has received his Ph.D. in analytical chemistry from University of South
Florida (USF), Tampa, Florida, USAwith specialization in sol-gel synthesis. He has invented 16-patented technologies in the area of chromatographic separation and
analytical/bioanalytical sample preparation. He has also authored/co-authored 9 book chapters, 6 review articles, 46 research articles and 89 conference papers.
akabir@fiu.eduAbuzar Kabir
Florida International University, Florida
Abuzar Kabir, J Anal Bioanal Tech 2018, Volume 9
DOI: 10.4172/2155-9872-C1-026