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Volume 5, Issue 3 (Suppl)
Mod Chem Appl, an open access journal
ISSN: 2329-6798
Global Chemistry 2017
September 04-06, 2017
September 04-06, 2017 | London, UK
5
th
Global Chemistry Congress
Mod Chem Appl 2017, 5:3(Suppl)
DOI: 10.4172/2329-6798-C1-006
Chemical constituents from the rhizomes of Curcuma zedoaria and assessment of their biological
activities The influence of preparation methods on halloysite nanotubes supported Ni catalysts for
hydrogenation of benzene
Omer Abdalla Ahmed Hamdi
Alneelain University, Sudan
P
hytochemical investigation of C. zedoaria resulted in the isolation of 21 compounds. Isolated compounds includes
eighteen sesquiterpenes and three labdane diterpenes. Various chromatographic techniques were used for the detection
and isolation of the compounds. Extensive spectroscopic methods including NMR, IR, UV, GC-MS, LC-MS were used for the
identification of the isolated compounds. Isolated compounds were subjected to cytotoxicity, anti-oxidant and neuroprotective
assays. Curcumenol and dehydrocurdione showed the highest protection (100%) against hydrogen peroxide induced oxidative
stress in NG108-15 cells at the concentrations of 4 and 8 µM, respectively. In the oxygen radical antioxidant capacity assay,
zerumbone epoxide showed the highest antioxidant activity with a Trolox equivalent (TE) of 35.41 µM per 100 µg of sample.
In the MTT based cytotoxicity assay against four cancer cell lines (Ca 41 Ski, MCF-7, PC-3 and HT-29), curcumenone and
curcumenol displayed strong antiproliferative activity (IC50 8.3 and 9.3µg/ml, respectively). A quantum chemical study was
performed to investigate their relationship with cytotoxic activity and revealed that the dipole moment (µ), molecular volume
(V), molecular area (A), polarizability (α) and hydrophobicity (log P) are the most important descriptors that influence the
cytotoxic activity of the compounds under investigation. The two most active compounds; curcumenol and curcumenone were
investigated for their binding to human serum albumin (HSA). The spectroflurometric analysis, in conjunction with molecular
docking study suggested that both curcumenol and curcumenone could bind to binding sites I and II of HSA with intermediate
affinity while site I was the preferred binding site for both molecules.
omerhamdi2001@hotmail.com