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Gastro 2016
August 11-12, 2016
Volume 6, Issue 4(Suppl) J Gastrointest Dig Syst 2016
ISSN:2161-069X JGDS, an open access journal
conferenceseries .com
August 11-12, 2016 Birmingham, UK
6th Global Gastroenterologists Meeting
O. O. Omotosho et al., J Gastrointest Dig Syst 2016, 6:4(Suppl)
Gastric lesions and molecular evidence of Helicobacter suis infection in pigs in Nigeria
O. O. Omotosho, B O Emikpe, O T Lasisi, O A Fagbohun, A A Owoade, O O Odupitan and T I Durotoye
University of Ibadan, Nigeria
Gastric lesions inpigs and humans have been associatedwithHelicobacter suis infection.This studywas designed to determine
the occurrence of gastric lesions and Helicobacter suis infection in pigs in Nigeria. Stomach from 480 pigs in Southwestern
Nigeria was assessed for gastric lesions using a modification of a standard lesion grading method. Mucosa samples from the
fundus of 160 pig stomachs from four regions of Nigeria (Lagos, Delta, Enugu and Plateau states) were collected for molecular
detection of H. suis. The DNA from samples was extracted with ZR Fungal/Bacterial DNA MiniPrep TM Isolation Kit (Zymo
research corp. USA). PCR was done using previously published primers. Data obtained were presented as frequency counts
and analysed using analysis of variance (ANOVA) and Chi-square techniques. Significance was determined at p≤0.05. Gastric
lesions were encountered across the four regions of the stomach with a point prevalence of 57.3%. The prevalence of lesions in
the non-glandular region was 32.9%. Helicobacter suis was detected in samples from all regions with frequency of occurrence
8%, 6%, 10% and 14% in Lagos, Delta, Enugu and Plateau states respectively. The gastric lesion distribution across the four
regions of the stomach and the occurrence of ulceration in the fundus showed an unusual pattern which is rarely reported in
other parts of the world. This is a first report of Helicobacter suis infection of pigs in Nigeria. These findings present the need
for further studies to determine its possible role in gastric lesions in pigs and humans in Nigeria.
Biography
O. O. Omotosho is a Lecturer and Doctoral student at the Department of Veterinary Medicine, University of Ibadan, Nigeria. He is Sub-Dean (Undergraduate) of
the Veterinary Faculty. He has conducted several researches in the field of Swine health, Infectious diseases of Swine and Gastroenterology. He has published 10
papers in reputed journals and is a reviewer to 3 journals.