gastro-congress-2016_posters-accepted-abstracts

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Volume 6, Issue 7(Suppl) J Gastrointest Dig Syst 2016
ISSN: 2161-069X JGDS, an open access journal
Page 56
Notes:
Gastro Congress 2016
October 24-25, 2016
conferenceseries.com
October 24-25, 2016 Valencia, Spain
9th Euro Global
Gastroenterology Conference
The profile of mealtonin receptors gene expressions and genes associated with their acivity in
colorectal cancer patients
P Kozieł1, K Walkiewicz 1, A Skubis2, B Sikora2, J Szota-Czyż2, C Kruszniewska-Rajs2, U Mazurek2, M Muc-Wierzgoń1
Medical University of Silesia, Poland
The antiproliferative and immunomodulatory effects of melatonin (MLT) have been demonstrated in a variety of neoplasms
including colorectal cancer (CRC). In humans and other mammals, MLT acts on target tissues through membrane and
retinoid nuclear receptors.The aimof the studywas to evaluate transcription activity of melatonin receptors and genes associated
with regulation of their activity in colorectal adenocarcinoma tissues in relation to clinical stage of cancer. A total of 24 pairs
of surgically removed tumoral and healthy (marginal) tissues samples from colorectal cancer patients at clinical stages I-II and
III-IV were collected. As the additional control, twenty normal samples were taken from people whose large intestine tissues
were reported as non-tumoral after colonoscopy. Expression of mRNA genes was studied by microarray HG-U133A analysis.
The analysis of genes expression profile was performed using commercially available oligonucleotide microarrays of HG-
U133A (Affymetrix, Santa Clara, CA). We found a high increase of MT1 mRNA expression levels in all cancerous samples vs.
non-cancerous tissues. The MT2 mRNA expression levels increased slightly in marginal and malignant samples. The melatonin
receptors gene expression was dependent on tumour grade. Among the genes participating in the cascade of signal transfer
in cell activated by MLT via MLT1 and MLT2, the following ones: GNA11, OXTR, and TPH1 encoding genes respectively as
differentiating stage III and IV of CRC. Monitoring the expression levels of genes that are related to melatonin receptors may
offer a strategy to anticipate tumour development and estimate the molecular changes that occur during carcinogenesis. The
mechanism behind this association needs further elucidation.
Biography
P Kozieł has graduated from Silesian Medical University in 2011 and works in Department of Internal Medicine in Bytom as Junior Assistant. Currently, he is also
a PhD student in the Medical Doctor program at Silesian Medical University in Katowice. His works were recently published in 4 international journals and 2 local
journals, in the areas of gastroenterology, oncology and endoscopic techniques in particular.
Paweł Kozieł et al., J Gastrointest Dig Syst 2016, 6:7(Suppl)