gastro-congress-2016_posters-accepted-abstracts

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Volume 6, Issue 7(Suppl) J Gastrointest Dig Syst 2016
ISSN: 2161-069X JGDS, an open access journal
Page 52
Notes:
Gastro Congress 2016
October 24-25, 2016
conferenceseries.com
October 24-25, 2016 Valencia, Spain
9th Euro Global
Gastroenterology Conference
Interleukin-1 receptor antagonist knockout mice as a model of the inflammatory bowel disease
Rasha Hatem Saeed Dosh
Sheffield Hallam University, UK
The inflammatory cytokine Interleukin 1 (IL-1) is an important mediator of inflammation and tissue damage in inflammatory
bowel disease (IBD). The activity of IL-1 is inhibited by a natural inhibitor: interleukin 1 receptor antagonist (IL-1Ra). The
balance between IL-1 and IL-1Ra plays a vital role in diseases. We investigated whether inflammatory bowel disease could be
induced spontaneously by the removal of IL-1Ra in mice. Histological staining was performed on BALB/Cmice to characterize
the morphology and enzyme activity of the small intestine from different ages and genotypes. Wild type mice served as a
negative control. 20 well oriented villi/crypt units and villus width at midvillus in longitudinal tissue sections were measured
in the jejunum and ileum. The number of goblet cells per villi was determined. Immuno histochemical staining was performed
to localise and detect MUC2, MUC5AC, MMP2, MMP9, ADAMTS1, IL-1β and TNFα. The results showed that there was a
significant decrease in the villi/crypts units’ height in the jejunum and ileum whereas the width of the villi was increased in the
jejunum and decreased in the ileum. The number of goblet cells per villi was increased in knockout mice compared with wild
type mice. Research is ongoing for the analysis of the immunohistochemistry. We conclude that IL-1Ra knockout mice could
act as a model for inflammatory bowel disease highlighting the importance of IL-1 in this disorder.
Biography
Rasha Hatem Saeed Dosh has completed her MSc from Al-Mustansiriyah University and worked as a Lecturer at University of Kufa College of Medicine/Iraq. She
has published 4 papers in college of medicine journals. She is currently a second year PhD student at Sheffield Hallam University/UK.
Rasha Hatem Saeed Dosh, J Gastrointest Dig Syst 2016, 6:7(Suppl)