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.com
Volume 7, Issue 5 (Suppl)
J Forensic Res 2016
ISSN: 2157-7145, JFR an open access journal
Forensic Research 2016
October 31-November 02, 2016
October 31-November 02, 2016 San Francisco, USA
5
th
International Conference on
Forensic Research & Technology
Study on comparison of DNAdetection rates in fire debris according to fire exposure temperature-time and distance
to Ignition point
Yang Jung Kim
National Forensic Service, South Korea
D
NA profiles from evidence found at crime scenes can be instrumental to identify a criminal in criminal investigations. But the
key evidence from crime scenes often contains very little DNA or degraded DNA by heat and light. Fire investigation is the
examination of fire-related incidents and similar to the examination of crime scenes. Fire investigation also includes surveying the
damaged scene to establish the origin of the fire and eventually establishing the cause and the fire whether or not crime-related. It
is difficult to determine whether arson has occurred because fire debris including the key evidence of fire origin is often seriously
damaged or contaminated by fire fighting water, fire extinguisher besides fire. To clarify the correlation of DNA detection rates and
evidence collection rates according to fire exposure temperature-time and distance to ignition point, in this study, we first analyzed
fire investigation cases including arson cases in Busan metropolitan city where 350 million people live. For this purpose, we also
conducted fire simulation experiments of DNA evidence samples. In addition, we performed a quantitative PCR (qPCR) assay to
quantify total DNA and the extent of DNA degradation and we compared detection rates of STR profiles of blood, saliva samples in
the fire exposure temperature condition at 100°C to 800
0
C and distance to ignition point from 1 m to 3 m. STR profiles of blood sample
were obtained in the fire exposure condition at 300
0
, 10 sec to 60 sec. STR profile has not been detected or evidence disappeared in
the fire exposure condition above 300
0
C, 60 sec. The distance to ignition point is farther, more complete STR profiles and evidences
were obtained.
Biography
Yang Jung Kim is the Chief of Busan Forensic DNA Smart Lab, National Forensic Service. She has completed her Master’s degree from Busan National University
and has worked for more than 15 years at Forensic DNA Division, National Forensic Service.
nalajmlee@gmail.comYang Jung Kim, J Forensic Res 2016, 7:5(Suppl)
http://dx.doi.org/10.4172/2157-7145.C1.021