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Volume 7, Issue 5 (Suppl)

J Forensic Res 2016

ISSN: 2157-7145, JFR an open access journal

Forensic Research 2016

October 31-November 02, 2016

October 31-November 02, 2016 San Francisco, USA

5

th

International Conference on

Forensic Research & Technology

Study on comparison of DNAdetection rates in fire debris according to fire exposure temperature-time and distance

to Ignition point

Yang Jung Kim

National Forensic Service, South Korea

D

NA profiles from evidence found at crime scenes can be instrumental to identify a criminal in criminal investigations. But the

key evidence from crime scenes often contains very little DNA or degraded DNA by heat and light. Fire investigation is the

examination of fire-related incidents and similar to the examination of crime scenes. Fire investigation also includes surveying the

damaged scene to establish the origin of the fire and eventually establishing the cause and the fire whether or not crime-related. It

is difficult to determine whether arson has occurred because fire debris including the key evidence of fire origin is often seriously

damaged or contaminated by fire fighting water, fire extinguisher besides fire. To clarify the correlation of DNA detection rates and

evidence collection rates according to fire exposure temperature-time and distance to ignition point, in this study, we first analyzed

fire investigation cases including arson cases in Busan metropolitan city where 350 million people live. For this purpose, we also

conducted fire simulation experiments of DNA evidence samples. In addition, we performed a quantitative PCR (qPCR) assay to

quantify total DNA and the extent of DNA degradation and we compared detection rates of STR profiles of blood, saliva samples in

the fire exposure temperature condition at 100°C to 800

0

C and distance to ignition point from 1 m to 3 m. STR profiles of blood sample

were obtained in the fire exposure condition at 300

0

, 10 sec to 60 sec. STR profile has not been detected or evidence disappeared in

the fire exposure condition above 300

0

C, 60 sec. The distance to ignition point is farther, more complete STR profiles and evidences

were obtained.

Biography

Yang Jung Kim is the Chief of Busan Forensic DNA Smart Lab, National Forensic Service. She has completed her Master’s degree from Busan National University

and has worked for more than 15 years at Forensic DNA Division, National Forensic Service.

nalajmlee@gmail.com

Yang Jung Kim, J Forensic Res 2016, 7:5(Suppl)

http://dx.doi.org/10.4172/2157-7145.C1.021