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conferenceseries.com
Volume 7, Issue 5 (Suppl) J Forensic Res 2016
ISSN: 2157-7145, JFR an open access journal Forensic Research 2016
October 31-November 02, 2016
October 31-November 02, 2016 San Francisco, USA
5th International Conference on
Forensic Research & Technology
Mass spectrometry-based forensic “Omics” in direct identification of body fluid protein markers
Sathisha Kamanna, Adrian Linacre, Nico Voelcker, Julianne Henry and Paul Kirkbride
Flinders University, Australia
Body fluids such as blood, seminal fluid, urine or saliva are very important in the investigation of crimes against the person such
as murder and rape. Whereas DNA profiling is extremely reliable in establishing from whom the body fluid originated, tests to
positively identify the type of fluid involved (e.g., whether it is semen or saliva or a mixture of them) are much less refined and can be
ambiguous. Our recently submitted article describes a streamlined and simplified direct approach for the identification of body fluids
using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF-MS) that avoids pre-fractionation
or isolation of proteins. Microliter quantities (or less) of neat fluids or their extracts or deposits of them in situ on tufts of fibers
plucked from evidence (such as garments) can be analysed directly and quickly. Here we describe extensions of our direct approach
in regards to the examination of other fluids, both human and non-human, and explore its combination with analysis of miRNA.
Biography
Sathisha Kamanna is pursuing 3rd year PhD at Flinders University, South Australia. His PhD project is “Mass Spectrometry-based proteomics applications
in forensic body fluids analysis”. He has 7 years work experience in biological mass spectrometry and is involved in the forensic analysis of body fluids and
identification of protein/miRNA biomarkers using mass spectrometry based analytical techniques. He has 8 international publications (author/co-author) in reputed
journals.
Sathisha Kamanna et al., J Forensic Res 2016, 7:5(Suppl)