euro-toxicology-2016_scientifictracks-abstracts

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Volume 6, Issue 6(Suppl) J Clin Toxicol 2016
ISSN: 2161-0495, JCT an open access journal
Page 122
Notes:
Euro Toxicology 2016
October 24-26, 2016
conferenceseries.com
Toxicology & Applied Pharmacology
October 24-26, 2016 Rome, Italy
7th Euro-Global Summit on
Genotypic and phenotypic patterns of antimicrobial susceptibility of Helicobacter pylori strains
among Egyptian patients
Marwa Saad Fathi
Ain Shams University, Egypt
Backgrounds & Study Aim: Helicobacter pylori is currently recognized as one of the most common chronic bacterial infections
worldwide. Eradication of bacteria is effective in healing peptic ulcers, preventing ulcer relapses and potentially decreasing the
risk of progression to gastric carcinoma. For successful eradication of bacteria, it is imperative that the clinician be aware of
the current antimicrobial susceptibility profiles of isolates within the region. Therefore, the aim of this study is to compare the
phenotypic & genotypic patterns of antibiotics susceptibility to Helicobacter pylori strains among Egyptian patients in order to
attain a clinical utility from such patterns.
Patients & Methods: 30 symptomatic cases were enrolled. H. pylori infection was diagnosed by upper endoscopy as well as
biopsy was taken. Antimicrobial susceptibility to Helicobacter pylori strains was assessed in all subjects by disc diffusion &
e-testing methods. Further molecular characterization for genes encoding antimicrobial resistance of isolated strains was done.
Results: For metronidazole, amoxicillin and ciprofloxacin, we compared the phenotypic and genotypic patterns of resistance as
detected by PCR amplification of the resistance genes. E test results were 100%, 50% & 87.5% for metronidazole, ciprofloxacin
& amoxicillin respectively from 16 isolated H. pylori strains.
Conclusion: Improving the knowledge of resistance mechanisms, the elaboration of rational and efficacious associations for
the treatment H. pylori infection are of high importance especially in determining the therapeutic outcome. Further progress
should ultimately focus on the establishment of a cheap, feasible and reliable laboratory test to predict the outcome of a
therapeutic scheme
Biography
Marwa Saad Fathi currently works as an Assistant Professor of Medical Microbiology & Immunology at Faculty of Medicine, Ain Shams University. She had her MD
degree from Ain Shams University in 2009. She is currently studying a Specialized Diploma in Medical Microbiology at University College, London. She is working
as a Director of Medical Mycology Lab at Misr University for Science & Technology (MUST). Her publications exceed 20 papers in important focused journals since
2009.
Marwa Saad Fathi, J Clin Toxicol 2016, 6:6(Suppl)