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.com
Volume 7, Issue 4 (Suppl)
J Clin Exp Pathol, an open access journal
ISSN: 2161-0681
Euro Pathology 2017
August 02-03, 2017
13
th
EUROPEAN PATHOLOGY CONGRESS
August 02-03, 2017 Milan, Italy
High expression of HIF1α is a predictor in patients with gastric adenocarcinoma
Rana Ezzeddini*, Mohammad Taghikhani*, Mohammad Hossein Somi**
and
Amir Salek Farrokhi***
* Department of Clinical biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
** Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
*** Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Background:
Gastric adenocarcinoma has been known as one of the virulent diseases with weak prognosis. New findings of gastric
cancers according to histologic features and molecular phenotypes improve early diagnosis, prevention and treatment. Hypoxia
inducible factor 1α (HIF1α) are expressed in some human cancers and were previously suspected of promoting tumor growing and
poor patient outcome. The objective of this study is to investigate the expression of HIF1α protein in adenocarcinoma tissues.
Materials & Methods:
We selected 105 gastric cancer patients and 130 healthy control with the age range of 30-80 in order to carry
out this study. The ethical committee of Tarbiat Modares University (Tehran, Iran) authorized this study. The study was explained
to the participants and Informed consent was obtained from all individuals. Formalin-fixed, paraffin wax-embedded sections were
cut at approximately 5 μm. Immunohistochemical staining for HIF1α were performed in all samples. Image J software was used for
microscopic investigation and comparing the outcomes. We used t test for data analysis. P values less than 0.05 was considered as
statistically significant difference in all cases.
Results:
Our findings indicated significant upregulation of HIF1α expression in gastric adenocarcinoma tissues (0.25±0.07) compare
to control group (0.18±0.04) ( P = 0.0001).
Conclusion:
As a result, the present study suggested that increased HIF1α were involved in progression of gastric adenocarcinoma
cells that can be used for distinguished classification.
Biography
Rana Ezzeddini is a PhD student in Clinical Biochemistry at Tarbiat Modares University. She received her master degree in Clinical Biochemistry, as well. Her main
research interest is clinical laboratory trials, with a focus on addressing biochemical questions for cancer and genetic deficienses.
rana.ezzeddini@yahoo.comRana Ezzeddini et al., J Clin Exp Pathol 2017, 7:4(Suppl)
DOI: 10.4172/2161-0681-C1-037