euro-pathology-2017-posters-accepted-abstracts

7 / 19

Page 69
Notes:
conferenceseries.com
Volume 7, Issue 4 (Suppl) J Clin Exp Pathol, an open access journal
ISSN: 2161-0681 Euro Pathology 2017
August 02-03, 2017
13 th EUROPEAN PATHOLOGY CONGRESS
August 02-03, 2017 Milan, Italy
Acanthamoeba secretes extracellular aminopeptidases to target cells for phagocytosis by disruption
Wei-Chen Lin
National Cheng Kung University, Taiwan
Acanthamoeba is free-living protist pathogen capable of causing a blinding keratitis and granulomatous encephalitis.
However, the pathogenesis mechanisms of Acanthamoeba are still not clear. Here, our results show that the rat glial
C6 cells co-cultured with Acanthamoeba would be spherical and floated, even without contact the protists. And then, the
Acanthamoeba protists would contact and engulf these cells. In order to clarify the contact-independent pathogenesis
mechanism in Acanthamoeba, we collected the Acanthamoeba secreted proteins (Asp) to incubate with cells for identifying
the extracellular virulent factors and investigating the cytotoxic process. Pre-treating the Asp with aminopeptidase inhibitor
and the specific antibodies of Acanthamoeba aminopeptidase could delay the progression of cell disruption during Asp and
cells co-incubation. Overall, these results suggest an important functional role of the Acanthamoeba secreted extracellular
aminopeptidases in Acanthamoeba pathogenesis process. This study provides new perspectives for the study of the mechanism
involved in the Acanthamoeba infection.
Biography
Wei-Chen Lin has completed his PhD from Chang Gung University and postdoctoral studies in the Center of Disease Control, Taiwan. Dr. Lin is one of the pioneers
on studying miRNA gene regulatory network in protozoan. His major research interest is to use bioinformatics approaches to integrate data generated by high-
throughput technologies to compare the gene, protein and miRNA expression levels of protozoan as a basis to elucidate the interactome of pathogen-host.
Wei-Chen Lin, J Clin Exp Pathol 2017, 7:4(Suppl)
DOI: 10.4172/2161-0681-C1-038