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Volume 5, Issue 6(Suppl) J Infect Dis Ther, an open access journal
ISSN: 2332-0877 Euro Infectious Diseases 2017
September 07-09, 2017
September 07-09, 2017 | Paris, France
Infectious Diseases
6th Euro-Global Conference on
Molecular characterization of extended spectrum beta-lactamases producing Enterobacteriaceae
causing lower urinary tract infection in pediatric population
Eltai N Omer
Qatar University, Qatar
Urinary Tract Infections (UTIs) continue to be the one of the most common cause of infections in pediatric patients in the
community. It is important to identify significant trend on anti-microbial resistance that may influence empirical treatment
& antibiotic stewardship. This study was designed with an objective of determining the prevalence of resistance mechanisms
in ESBL producing Enterobacteriaceae isolated from pediatric patients attending Pediatrics Clinic (PEC) Al Saad, Qatar. The
isolates were identified by MALDI-TOF and phenotypic antimicrobial susceptibility testing was performed by BD Phoenix and
confirmed by double disk synergetic test (DDST). PCR and multiplex PCR-were performed for molecular characterization of
different groups of ESBL. Out of a total of 566 positive urine cultures, E. coli (84%) was the most predominant uropathogen
followed by K. pneumoniae (11%) and 197 (34.8%) were found to be ESBL producing Enterobacteriaceae isolates. Male to
female ratio was 1: 4.7. Of these positive ESBL isolates, 119 were included in our study with E. coli being the pre major dominant
isolate 104 (87.4%), followed by K. pneumonia 13 (11%) then E. cloacae 1(0.8%) and C. koseri 1 (0.8%). TXM was found to be
the gene responsible for 63% of ESBL, followed by TEM 23.5 then a combination of TEM and SHV 9.2% and 4.2% were due
to SHV. In conclusion, to our knowledge, there are no published data on UTI etiological agents and their analogues genotypic
characteristics of resistant species of bacteria among children in Qatar. Our findings generate crucial information about the
molecular epidemiology of resistant Gram-negative bacteria in pediatric population in Qatar. Accordingly, it will help in
understanding the ESBLs dynamic and associated risk factors. More importantly it will help in establishing the anti-microbial
stewardship program in Qatar and limiting the spread of antibiotic resistant bacteria in the community by implementing the
evidence based infection control measures.
Biography
Eltai N Omer has completed her PhD from Humboldt University, Berlin, Germany and Postdoctoral studies from University of the West of England, UK. She is
Research Associate at Biomedical Research Centre, Qatar University. She has published many papers in the field of Antibiotic Resistance. Her research interests
are multidisciplinary with emphasis on molecular diagnostic approaches, antimicrobial susceptibility & resistance, test of new natural antimicrobial agents. She is
adopting the one health system approach by studying antimicrobial resistance in agriculture, environment and human in Qatar.
Eltai N Omer, J Infect Dis Ther 2017, 5:6(Suppl)
DOI: 10.4172/2332-0877-C1-033