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conferenceseries
.com
Volume 5, Issue 6(Suppl)
J Infect Dis Ther, an open access journal
ISSN: 2332-0877
Euro Infectious Diseases 2017
September 07-09, 2017
September 07-09, 2017 | Paris, France
Infectious Diseases
6
th
Euro-Global Conference on
Prevalence of Aerobic Gram-Negative Bacilli in Lower Respiratory Tract Infections in Menoufia
Governorate, Egypt
Rabab A. Elwahsh, Shymaa A. El Askary, Amal F. Makled, Gehan A. Abdel Aal
and
Reda A. Ibrahem
Menoufia University, Egypt
Background:
Lower Respiratory Tract Infections (LRTIs) are among the most common infectious diseases affecting humans
worldwide and are considered as an important cause of morbidity and mortality for all age groups. Almost three quarters of all
antibiotic consumptions are for respiratory tract infections.
Methods:
Two hundred and twenty two gram negative bacteria (GNB) were isolated from 763 LRTIs specimens in the period
from February 2015 to January 2016 by conventional microbiological methods. Multidrug-resistance (MDR), extensively
drug-resistance (XDR) and pan drug resistance (PDR) for GNB were examined by disc diffusion method. ESβL and MβL GNB
suspected strains were studied by screening and confirmatory tests.
Results:
The prevalence of culture positive specimens was (65.9%) of the studied specimens, 44.1% of them were aerobic
GNB which was distributed as 35.8% of the ward isolates and 60.7% of ICUs isolates. Klebsiella spp. (44.6%) was the most
common GNB isolated from LRTIs patients followed by E coli (20.3%), Pseudomonas spp. (18%), Acinetobacter spp. (10.8%),
Enterobacter (4.5%) and Citrobacter (1.8%). Total MDR, XDR and PDR GNB were 45.5%, 47.8% and 5.0% respectively. There
was statistically significant difference between the studied fermentative GNB and non-fermentative GNB (60.1% Vs. 42%) for
ESβL production by Cephalosporin/clavulanate combination disks test (confirmatory test). The highest percentage of MβL
production by confirmatory IPM/EDTA was for Acinetobacter spp. (62.5%) followed by Pseudomonas spp. (60%), Klebsiella
spp. (52.5%) and E coli (40%). The mortality rate was 7.4% and 10.9% in patients who had ESβL or MβL producing isolates
respectively.
Conclusions:
Multidrug-resistant (MDR) gram-negative bacilli (GNB) are now widespread especially in patients with LRTIs
and present a major challenge tomodernmedical practice. Longer hospital stay, ICU admission, invasive procedures, associated
comorbid conditions and empirical antibiotic usage were significantly high risk factors for acquisition of ESβL and MβL.
Biography
Rabab El wahsh and her coworkers has conducted this study to determine the prevalence of aerobic gram negative bacteria among LRTIs patients and associated
risk factors in addition to its effect on patient outcome with declaration of MDR aerobic Gram-negative bacilli (GNB) causing LRTIs, with a special reference to
extended-spectrum beta-lactamase(ESβL) and metallo-beta-lactamase (MβL) producing bacterial strains and to study their relation with patient’s mortality and
morbidity. They found Multidrug-resistant (MDR) gram-negative bacilli (GNB) are now widespread especially in patients with LRTIs and present a major challenge
to modern medical practice.
rababwahsh@yahoo.comRabab A. Elwahsh et al., J Infect Dis Ther 2017, 5:6(Suppl)
DOI: 10.4172/2332-0877-C1-033