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.com
Volume 8, Issue 3 (Suppl)
J Clin Cell Immunol, an open access journal
ISSN: 2155-9899
Euro Immunology 2017
June 29-July 01, 2017
June 29-July 01, 2017 Madrid, Spain
8
th
European
Immunology Conference
Vanadium and chromium mediated impairments in the immunological reactivity of rats with aseptic
inflammation
Aliya N. Tokusheva
1
, Marina K. Balabekova
2
, Yekaterina O. Ostapchuk
3
, Nikolay N. Belyaev
3
and
Rustam R. Tukhvatshin
4
1
Asfendiyarov Kazakh National Medical University, Kazakhstan
2
Asfendiyarov Kazakh National Medical University, Kazakhstan
3
M.A.Aitkhozhin’s Institute of Molecular Biology and Biochemistry, Kazakhstan
4
I.K. Akhunbaev Kyrgyz State Medical Academy, Kyrgyzstan
C
hronic inflammation is a consequence of the immune system dysfunction. Such dysregulation of the immune response may
induce chronic diseases, such as autoimmune diseases, diabetes, and malignant transformation of cells. Xenobiotics, including
vanadium and chromium, were shown to induce inflammatory changes leading to chronic inflamation. The purpose of this research
was to study the process of aseptic inflammation accompanied with intoxication with salts of heavy metals. In our study, sexually
mature rats were administered aseptic inflammation (AI) alone or ammonium vanadate and potassium dichromate (AV/PD) at a
dose of 5 mg/kg of BW for two weeks and after that aseptic inflammation was modeled. Lymphatic organs were studied on day 1, 7,
and 14 after the onset of aseptic inflammation. Administration of AV/PD and AI resulted in structural changes in lymphatic organs
and anemia observed throughout the experiment. We observed a decrease in the cellularity of the bone marrow and thymus, ratio
of thymic cortex to medulla, and dystrophic changes in thymic cells and their scarcity. Also, we detected increased levels of anti-
inflammatory cytokines in serum: IL-10 on day 1 and TGF-β on day 7 and 14 after the beginning of the experiment in the group
AI+AV/PD comparing to intact rats and AI group. Phenotypical analysis demonstrated that by the end of experiment freshly obtained
splenocytes of AI and AI+AV/PD rats contained increased percentage of His48
+
CD11b/c
+
and His48
high
CD11b/c
+
cells, and decreased
number of induced CD3
+
CD4
+
IFNγ
+
, CD3
+
CD4
+
IL-4
+
and CD3
+
CD8
+
cells, comparing to control animals. Interestingly, during the
next period of the experiment, we observed significant decrease of induced CD3
+
CD4
+
IFNγ
+
, CD3
+
CD4
+
IL-4
+
and CD3
+
CD8
+
cells
in the AI+AV/PD group comparing to AI group.
Thus, it is possible that an elevated sera IL-10 and TGF-β1 observed in mice with chronic inflammatory processes administered with
AV/PD result of abundant accumulation of His48
+
CD11b/c
+
and His48
high
CD11b/c
+
cells myeloid cells in the periphery, and, in turn,
it could participate in the maintaining of the immunosuppressive environment that supports persistence of chronic inflammatory
conditions. Therefore, intoxication with vanadium and chromium salts may support immunosuppressive environment, contributing
to chronic inflammation development.
Biography
Aliya Tokusheva is a PhD student at the Asfendiyarov Kazakh National Medical University. She studies molecular mechanisms of epigenetic regulation of tissue-
specific expression of genes, revealing the effect of heavy metal compounds on the variability of the genome of immunogenesis organs and the mutual regulatory
influence of important links of these mechanisms on the course of the inflammatory process.
aliyatokusheva@mail.ruAliya N. Tokusheva et al., J Clin Cell Immunol 2017, 8:3(Suppl)
DOI: 10.4172/2155-9899-C1-037