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Volume 7, Issue 2 (Suppl)
J Adv Chem Eng, an open access journal
ISSN: 2090-4568
Euro Chemical Engineering 2017
November 16-17, 2017
ADVANCES IN CHEMICAL ENGINEERING AND TECHNOLOGY
November 16-17, 2017 | Paris, France
2
nd
International Conference on
Engineering of
Escherichia coli
to facilitate efficient utilization of isomaltose and panose in industrial
glucose feedstock
Kenji Abe
Ajinomoto Co. Inc., Japan
F
ermentative production of useful compounds, such as alcohols, gases, pharmaceutical ingredients, and organic/amino acids,
is conducted worldwide.
Escherichia coli
is one of the most useful bacterium for the production of valuable compounds,
such as amino acids and organic acids, because
E. coli
cells grow quickly, convert substrates to products rapidly, and are
genetically engineered readily. For example, L-lysine, which is used as a feed additive worldwide, is produced on the scale
of approximately 1,500,000 metric tons per year. On the other hand, in the industrial production of useful compounds by
fermentation, glucose is one of the most frequently used carbon sources. Industrial glucose feedstock prepared by enzymatic
digestion of starch typically contains significant amounts of disaccharides such as maltose and isomaltose, and trisaccharides
such as maltotriose and panose. Maltose and maltosaccharides can be utilized in
Escherichia coli
fermentation using industrial
glucose feedstock because there is an intrinsic assimilation pathway for these sugars. However, saccharides that contain α-1,6
bonds, such as isomaltose and panose, are still present after fermentation because there is no metabolic pathway for these
sugars. To facilitate more efficient utilization of glucose feedstock, we introduced glvA, which encodes phospho-α-glucosidase,
and glvC, which encodes a subunit of the phosphoenolpyruvate-dependent maltose phosphotransferase system (PTS) of
Bacillus subtilis, into
E. coli
. The heterologous expression of glvA and glvC conferred upon the recombinant the ability to
assimilate isomaltose and panose. The recombinant
E. coli
assimilated not only other disaccharides but also trisaccharides,
including alcohol forms of these saccharides, such as isomaltitol. To the best of our knowledge, this is the first report to show
the involvement of the microbial PTS in the assimilation of trisaccharides. Furthermore, we demonstrated that an L-lysine-
producing
E. coli
harboring glvA and glvC converted isomaltose and panose to L-lysine efficiently. These findings are expected
to be beneficial for industrial fermentation.
Figure1
.
Schematic illustaration of GlvAC pathway for assimilation of isomaltose (iMal) and panose (Pan).
Biography
Kenji Abe is an employee of Japanese company, Ajinomoto Co., Inc.. He has his expertise in industrial fermentation technology and biotechnology.
kenji_abe68@ajinomoto.comKenji Abe, J Adv Chem Eng 2017, 7:2(Suppl)
DOI: 10.4172/2090-4568-C1-002