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.com
Volume 7, Issue 5 (Suppl)
Epidemiology (Sunnyvale), an open access journal
ISSN: 2161-1165
Epidemiology 2017
October 23-25, 2017
EPIDEMIOLOGY & PUBLIC HEALTH
October 23-25, 2017 | Paris, France
6
th
International Conference on
Epidemiology (Sunnyvale) 2017, 7:5(Suppl)
DOI: 10.4172/2161-1165-C1-018
EMERGING DRUG RESISTANCE SALMONELLA STRAINS FROM CHICKEN MEAT
Nahed Ghoneim
a
and
MonaKadry
a
a
Cairo University, Egypt
T
his work was carried out to study the occurrence of salmonella infections in chicken and human suffering from
gastroenteritis in Egypt. A total of 349 chicken meat and 40 stool specimens of children were collected, samples
were subjected to bacteriological examination and identified biochemically as salmonella then subjected to serological
identification, also were tested for their antibiotic susceptibility by disc diffusion method Also, genotyping by PCR to detect
salmonella enterotoxine gene (stn)
and their expression by infant mouse assay. the study revealed that 14 (4.01%) and 2 (5%)
were positive for
Salmonella species
in chicken meat and children respectively and serological identification were
(Salmonella
Infantis, Salmonella Typhi, Salmonella Kentucky, Salmonella Rubislaw, Salmonella Poona, Salmonella Typhimurium, Salmonella
Virginia, Salmonella Enteritidis and Salmonella Montevideo)
and
(Salmonella Kentucky and Salmonella Enteritidis)
in chicken
meat and children isolates respectively. Disc diffusion method showed that 3 (21.4%) in chicken meat isolates and 2 (100%) in
children isolates were multidrug resistant in which S. Kentucky have resistance to ciprofloxacin, the drug of choice for treating
salmonellosis in children. Also, genotyping showed that 9 (64.28%) and 2 (100%) isolates confirmed to be enterotoxigenic
strains in chicken meat and children respectively and this (stn) gene have been expressed (100%) by infant mouse assay.
Sequencing and phylogenetic tree of 4 studied isolates resulted in assessment the relations between different isolation sources.
Special attention must be paid to antibiotics that are used exclusively in poultry farms, appropriate measures must be taken to
control the spread of resistant bacteria to human.