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Volume 7, Issue 1 (Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
March 20-21, 2017 Rome, Italy
&
15th World Congress on
2nd International Conference on
Biotechnology And Biotech Industries Meet
Enzymology and Molecular Biology
Enzymology & Mol. Biology 2017
Biotechnology Congress 2017
March 20-21, 2017
The role of zeolite in reducing oxidative damage in tomato plants exposed to drought
Dino Hasanagic1, Danijela Kojic2 and Biljana Kukavica1
1University of Banja Luka, Bosnia and Herzegovina
2University of Novi Sad, Serbia
Statement of the Problem: The drought is a worldwide problem and insufficient supply of plants with water is one of the most
important causes of low agricultural yields and thus representing one of the most common problems faced by the producers. There
has been an increased interest in science in recent years in the use of natural aluminosilicates in agriculture where the most famous
is zeolite, a mineral whose absorption properties and balanced release of water and nutritive substances ever more successfully solve
the issue of water supplying and mineral nutrition and have beneficial impact on overall plant growth. The aim of this study was
to investigate the role of zeolite in prevention of oxidative stress in tomato plants exposed to drought. Changes in the activity of
peroxidase (POD, EC 1.11.1.7), catalase (CAT, EC 1.11.1.6), ascorbate peroxidase (APX, EC 1.11.1.11), superoxide dismutase (SOD,
EC 1.15.1.1) as well as reduced and total ascorbate content in plant leaves exposed to drought for 28 days were investigated. Activities
of antioxidant enzymes in the leaves of plants exposed to drought were at the same level with and without the addition of zeolite.
The obtained results indicate that zeolite did not prevent oxidative damages caused by drought. Native electrophoresis resolved the
presence of two peroxidase isoforms specific for drought and their activities were higher in tomato leaves whit zeolite. The drought
induced an increase in activities of superoxide dismutase, ascorbate peroxidase and ascorbate concentration and this antioxidative
strategy was more expressed in zeolite treated plants. Unexpected results related to the role of zeolite open the possibility to different
perspectives in discussion on the zeolite role in drought prevention.
Biography
Dino Hasanagic received his degree of . from the Faculty of Science in Sarajevo, and he is currently attending plant biochemistry programme at Faculty of
Science in Novi Sad, Serbia. His field of interest is antioxidative metabolism of plant cell - effects of abiotic stress (drought, metals, senescence, ROS signaling,
redox state). Dr Biljana Kukavica is expert in plant enzyme antioxidative metabolism. Her interest is focused on enzyme and non-enzyme antioxidative defense
in plant cell under oxidative stress. Dr Danijela Kojic has a big experience in the field of biochemistry and molecular biology and her researches are addressing
the antioxidative enzyme role in adaptation to stress conditions. The expertise and passion of the authors is recognized among their peers, and they constantly
expanding their knowledge in researches regarding enzymology.
Dino Hasanagic et al., J Biotechnol Biomater 2017, 7:1(Suppl)