enzymology-and-biotechnology-congress-2017_tracks

3 / 31

Page 38
Notes:
conferenceseries.com
Volume 7, Issue 1 (Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
March 20-21, 2017 Rome, Italy
&
15th World Congress on
2nd International Conference on
Biotechnology And Biotech Industries Meet
Enzymology and Molecular Biology
Enzymology & Mol. Biology 2017
Biotechnology Congress 2017
March 20-21, 2017
Enzymatic synthesis of prebiotic galacto-oligosaccharide: Application of nanobiocatalysts and structural
characterization of product
Dejan Bezbradica
University of Belgrade, Serbia
Statement of Problem: Galacto-oligosaccharides (GOS) are group of β - galactoside compounds with significant market value due
to their prebiotic properties utilized in infant nutrition products. Physiological activity is based on their short chain carbohydrate
structure which makes them non-digestible by digestive enzymes, but digestible by beneficial probiotic bacteria with consequential
property of selective promotion of their growth and improvement of overall health status. State of the art in current industrial GOS
production based on transgalactosylation activity of β-galactosidases implies that attempts for further advance could be focused on:
Fine-tuning of physiological properties by targeted control of enzymatic process toward obtaining GOS of desired structure and
developing novel immobilized β-galactosidase preparations with improved affinity towards GOS synthesis.
Methodology & Theoretical Orientation: For evaluation of the effect of enzyme origin on degree of polymerization and type of
β-linkages within obtained GOS compounds, transgalactosylation was performed with different β -galactosidases: from Aspergillus
oryzae and Lactobacillus acidophilus . Elucidation of chemical structures in obtained GOS mixtures was performed using ion-
mobility spectrometry−tandem mass spectrometry (IMS-MS/MS) one-step approach. Improvement in the field of β-galactosidase
immobilization was attempted by producing novel nanobiocatalyst with functionalized nonporous fumed nano-silica (FNS) particles
as immobilization support.
Conclusion& Significance: IMS-MS/MS analysis has shown that structure of obtained GOS is influenced by origin of β-galactosidase,
since one from A. oryzae produced GOSs with β(1→6) and β(1→3) linkages, while enzyme from L. acidophilus produces GOSs with
β(1→6) and β(1→4) linkages. Type of glycosidic linkages influences prebiotic properties of GOS, hence determination of linkage type
will have great significance in enabling adequate selection of β-galactosidase for targeted prebiotic application. The immobilization
on nano-supports indicated that the most adequate support is one functionalized with amino groups, which enabled several times
higher transgalactosylation activities than conventionally immobilized β-galactosidase.
Biography
Dejan Bezbradica obtained his PhD degree in Biochemical Engineering and Biotechnology from the Faculty of Technology and Metallurgy in Belgrade in 2007.
Since 2013, he is an Associate Professor in the Department of Biochemical Engineering and Biotechnology. During 2009, he was on sabbatical working in the
Laboratory of Enzyme Engineering at Institute of Catalysis in Madrid. His scientific work covers following areas: Cell and enzyme immobilization, enzymatic
synthesis in microaqueous media, application of membrane reactors in biocatalytic processes; microbial production and purification of industrial enzymes, kinetic
modeling of bisubstrate enzymatic reactions, application of enzymes with transglycosylative activity in synthesis of bioactive compounds, chemical modification of
enzymes and immobilization supports, and nanobiocatalysis. His recent research activities are focused on the development of food and feed products containing
bioactive galactosides with prebiotic activities targeted for specific probiotic species.
Dejan Bezbradica, J Biotechnol Biomater 2017, 7:1(Suppl)