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conferenceseries.com
Volume 7, Issue 1 (Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
March 20-21, 2017 Rome, Italy
&
15th World Congress on
2nd International Conference on
Biotechnology And Biotech Industries Meet
Enzymology and Molecular Biology
Enzymology & Mol. Biology 2017
Biotechnology Congress 2017
March 20-21, 2017
Lactic acid production from hemicellulosic fraction of sorgum bagasse by Lactobacillus pentosus
Danielle da Silveira dos Santos Martins, Ludmila de A N Viana, Elcio R Borges, Edmar da M Penha and Nei Pereira Jr
Federal University of Rio de Janeiro, Brazil
Among the various renewable feedstocks available for bioproducts synthesis, the sweet sorghum stands out as one of the most
promising due to its wide adaptability to different types of climate and soil. Furthermore, it is the only crop that provides stalks
and grains which can be used in the food industry, and the exceedance biomass can be used such as second generation organic acids.
Most organic acids on the market are produced via chemical synthesis with high levels of pollution. The production of organic acids of
second generation is inserted in the biorefinery context, advancing towards emerging future technologies. In this context, lactic acid
is considered a commodity with multiple industry applications, as well the polylactic acid (PLA) syntesis. The aim of this work was
the preliminary study of the lactic acid production from hemicellulosic fraction of sorgum bagasse by Lactobacillus pentosus. Initially,
the bagasse was submitted to a pretreatment with diluted acid to fractionate and extract the hemicellulose component from the solid
residue named cellulignin. Batch fermentation experiments were performed under the principles of the statistical methodology of
response surfaces to define the optimum process conditions- inoculum, xylose and KH2PO4 concentrations- under the 37ºC, 120
rpm. The experiments were performed until a statistical model to study the effect of several variables and to seek optimum conditions
for a multivariable system. The Lactobacillus pentosus strains exhibited increased ability to uptake and ferment xylose, reaching until
20 g/L lactic acid production.
Biography
Danielle da Silveira dos Santos Martins has completed her PhD in Sciences from the Post-graduation Program on Technology in Biochemical and Chemical
Process from Federal University of Rio de Janeiro, Brazil. She is a Professor at Federal University of Rio de Janeiro, in Biotechnology with emphasis on Industrial
Microbiology and Fermentation. She participated in theoretical-experimental projects development, scientific research and development in bioprocesses. Her
subjects of research includes “The process development aimed at biofuels production, enzymes, biosurfactants, organic acids, waste treatment and industrial
effluents and molecular biology techniques development”.
Danielle da Silveira dos Santos Martins et al., J Biotechnol Biomater 2017, 7:1(Suppl)