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.com
Volume 7, Issue 2 (Suppl)
J Ecosyst Ecography, an open access journal
ISSN:2157-7625
September 18-20, 2017
September 18-20, 2017 Toronto, Canada
Joint Conference
International Conference on
International Conference on
Environmental Microbiology and Microbial Ecology
&
Ecology and Ecosystems
María del Pilar Angarita Díaz et al., J Ecosyst Ecography 2017, 7:2 (Suppl)
DOI: 10.4172/2157-7625-C1-030
Inhibition activity of probiotic supernatants against the cariogenic
Streptococcus mutans
María del Pilar Angarita Díaz
1
, Sidónio Ricardo da Cunha Freitas
1
, Alejandro Mira Obrador
2
, Claudia Maria Bedoya Correa
3
and
Alejandro Peláez Vargas
3
1
Universidad Cooperativa de Colombia, Colombia
2
Fundación para el Fomento de la Investigación Sanitaria y Biom dica, Spain
3
Universidad Cooperativa de Colombia, Colombia
Statement of the Problem:
Among the alternatives strategies to antibiotics are other antimicrobial substances like bacteriocins.
However, since their production and purification is laborious and costly, an attractive approach is the administration of probiotic
bacteria with bacteriocin-producing capabilities. These types of probiotic bacteria like
Lactobacillus rhamnosus
isolated from the
intestinal tract and
Streptococcus dentisani
isolated from the oral cavity have been studied for diseases control. Therefore, the aim of
this study was to determine the inhibitory effect of supernatants from
L. rhamnosus
and
S. dentisani
on
S. mutans
.
Methodology & Theoretical Orientation:
Broth cultures of
L. rhamnosus
G G and
S. dentisani
7746 were prepared with 100
nephelometric turbidity units and grown overnight to reach stationary phase. The cultures were centrifuged at 4000rpm for 10min,
and the supernatants recovered and filtered to remove any bacteria cells. 100µl of each supernatant were added to 100µl of fresh
culture of serotype c strains
S. mutans
UA159 and ATCC 25175 at 10
8
CFUs/ml in a 96-wells plate. Controls were 100µl of
S. mutans
strains with 100µl culture medium used for the growing of each probiotic bacteria (BHI for
S. dentisani
and MRS for
L. rhamnosus
).
The 96-wells plate was incubated at 37°C inside a UV/V are spectrophotometry reader and the absorbance was monitoring at 600nm
every 20minutes for 15h.
Findings:
The supernatants of
S. dentisani
7746 and
L. rhamnosus
GG present an inhibitory effect against the cariogenic strains
S.
mutans
25175 and UA 159. The inhibition with the supernatant of
L. rhamnosus
was immediate while with the supernatant of
S.
dentisani
was significant after 3h (p=0.038, T-student).
Conclusion & Significance:
The use of supernatants from probiotic bacteria
S. dentisani
and
L. rhamnosus
has an inhibitory effect
against two strains of cariogenic
S. mutans
. Further studies are recommended with different supernatant concentration.
Biography
María del Pilar Angarita Díaz has a degree in Microbiology and Ph.D in Biotechnology. The knowledge in these topics has allowed her to contribute in the Faculty of
Dentistry of the Universidad Cooperativa de Colombia, in different studies such as probiotics for caries prevention and control, and microbiologic analysis of dental
material. She teaches in the area of the oral microbiology, knowledge that is enriched with the research.
maria.angaritad@campusucc.edu.com