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Volume 7, Issue 2 (Suppl) J Ecosyst Ecography, an open access journal
ISSN:2157-7625 September 18-20, 2017
September 18-20, 2017 Toronto, Canada
Joint Conference
International Conference on
International Conference on
Environmental Microbiology and Microbial Ecology
&
Ecology and Ecosystems
Andrew Jamnik, J Ecosyst Ecography 2017, 7:2 (Suppl)
DOI: 10.4172/2157-7625-C1-030
The adaptation of the bacterial plant pathogen Pseudomonas syringae onto a novel host through
experimental evolution
Andrew Jamnik
University of Toronto, Canada
The bacterial plant pathogen Pseudomonas syringae is a highly diverse species complex, with the ability to cause disease on a wide
range of hosts, including many economically important crops. Although P. syringae as a species has a very large host range,
individual strains are highly host specific. The inability of a strain to infect plant species outside of its host range is generally due to its
inability to suppress and evade the host’s innate immunity. Few studies to date have tested how plant pathogens evolve to overcome
novel host immunity, and a further understanding of this could help in uncovering how newly emerging diseases arise. In this study,
we are currently experimentally evolving P. syringae pv. phaseolicola (Pph ) 1448A, a strain which causes disease on many cultivars of
the common bean (Phaseolus vulagris), on the non-host Arabidopsis thaliana (Arabidopsis) through in planta experimental evolution.
Although Pph 1448A is unable to cause disease on Arabidopsis, its ability to grow and persist on Arabidopsis has given this strain
the potential to adapt within this novel environment. We have constructed 12 uniquely barcoded hyper mutating lineages of Pph
1448A for this experiment, to increase the evolvability of the lineages and to allow us to study mutations associated with adaptation
towards Arabidopsis. By using highly sensitive in planta competition assays on Arabidopsis, we’ve shown that after 80 days of in planta
growth on Arabidopsis, two lineages have shown significant gains in fitness on the novel host. Additionally, one of these lineages has
shown a gradual increase in fitness throughout the experimental evolution. Population sequencing of these adapted lineages will
give us the ability to identify candidate mutations responsible for this novel host adaptation. Overall, this work will provide a further
understanding of the early adaptive processes underlying the spread of existing pathogens to new hosts.
Biography
Andrew Jamnik is currently a second year MSc candidate at the University of Toronto working under the supervision of Dr. David Guttman. His research has
focused on understanding the evolutionary steps a plant pathogen takes to overcome novel host immunity, and to cause disease on a new host. He has specifically
been working with the model bacterial plant pathogen Pseudomonas syringae and has designed and implemented in vivo experimental evolution to uncover the
evolutionary steps required for novel host adaptation. Throughout his graduate degree, he has learned both invaluable microbiology skills along with a better
understanding of evolutionary genomic analyses.