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Journal of Ecosystem & Ecography | ISSN: 2157-7625 | Volume 8
July 11-12, 2018 | Toronto, Canada
International Conference on
Environmental Microbiology & Microbial Ecology
International Conference on
Ecology, Ecosystems & Conservation Biology
&
Metagenomic detection of bacteria and fungi in the atmosphere of Mexico City
Serrano-Silva N, Calderón-Ezquerro
and
Brunner C
National Autonomous University of Mexico, Mexico
B
ioaerosols significantly affect atmospheric processes. The identification of airborne bacteria and fungi has traditionally
been performed by retrieval in culture media, but in this way their diversity in the air is underestimated. Advances in DNA
sequencing technology have produced a broad knowledge of genomics and metagenomics, so the objective of this study was
to efficiently recover microorganisms from the air and standardize monitoring protocols, sample processing and molecular
detection of bioaerosols of the Mexico City. To examine this bioaerosols we performed deep sequencing on the 16S rRNA
and ITS genes from air samples collected during ten weeks (February to May, 2016) in south of Mexico City. Three samplers
were used: a Durham-type spore trap (Durham), a seven-day recording volumetric spore trap (HST), and a high-throughput
'Jet' spore and particle sampler (Jet). A simple and efficient method for collecting bioaerosols and extracting good quality
DNA for deep sequencing was standardized. The most abundant bacteria phyla in the air were Actinobacteria, Proteobacteria
and Firmicutes. The HST sampler collected the largest amount of airborne bacterial and fungal diversity, however it may be
preferred to use one or the other sampler, as each one collected preferentially some groups, i.e. Durham favors the sampling
of Cyanobacteria and HST the sampling of Firmicutes. The most abundant fungal phyla in the air were Ascomycota and
Basidiomycota. Methods of sampling and processing of samples for metagenomic detection of bacteria and fungi in the air
were standardized, which allows a deep exploration of the diversity of airborne bacteria and fungi.
Biography
Nancy Serrano-Silva has her expertise in molecular methods to study microbial communities in environmental samples, mainly soils and air. Currently she is a
Posdoctoral Researcher in the Center of atmospheric sciences at National Autonomous University of Mexico. Serrano-Silva and Calderón-Ezquerro have been
working since 3 years ago in the implementation of metagenomic to evaluation of air quality (indoor and outdoor) in Mexico City. This is one of the first jobs that
report the use of equipment such as the Hirst spore trap and the Durham sampler to collect and identify the microbiota of the atmosphere by using a metagenomic
focus.
nserranos@gmail.comSerrano-Silva N et al., J Ecosys Ecograph 2018, Volume 8
DOI: 10.4172/2157-7625-C3-039