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Journal of Ecosystem & Ecography | ISSN: 2157-7625 | Volume 8
July 11-12, 2018 | Toronto, Canada
International Conference on
Environmental Microbiology & Microbial Ecology
International Conference on
Ecology, Ecosystems & Conservation Biology
&
Operational taxonomic units that distinguish soils of crops and fruits in the whole Japan
Andre F Cruz
1
, Atsushi Nakao, Junta Yanai, Tetsushi Tanaka
2
, Naoaki Ono
and
Shigehiko Kanaya
1
Kyoto Prefectural University, Japan
2
Nara Institute of Technology, Japan
T
he microbial community evaluation constitutes an essential topic to access the soil health conditions, with special regard
to the operational taxonomic units (OTU). This data can easily represent the soil bacterial condition and also might
distinguish many kinds of environment, such as urban areas, and agricultural soils. As a criterion to set up a “finger-print” for
agricultural soils in terms of microbial community, metagenomic profiles from crops and fruit soils had their OTU analyzed
after the normalizing their abundance where the sum of each sample was equivalent to 1. The bacterial and fungal genes
were the target region evaluated by principal component analysis (PCA). Although some dominant taxonomic groups were
constantly present in most of the soils, the OTU within the bacterial and fungal communities were specific according to the
land use type (crops or fruits) i.e. each one had a remarkable difference in terms of OTU. Hokkaido prefecture contained most
of specific groups, whereas other prefectures were more diverse. Similar profile was found in fungal genes. Within the bacterial
genes a few number of OTUs made possible to separate crops and fruit soils, however for the fungal ones a large number
was necessary for the same goal. The bacterial representative genera were
Nitrospira
(involved with Nitrogen cycling) and
Arthrobacter
. Whereas the
Fusarium
was the most important fungus in terms of OTU bioindicator.
Biography
Andre Freire Cruz had focused on evaluation of soil microbial status as indicator of sustainability. He has worked with many agricultural soils, recently has
concentrated on fruit orchards in Japan and other countries especially concerning on analysis of microbial growth and activity. He has used many methods to
evaluate the soil biology, but actually most of his researches are using metagenome analysis of bacterial and fungal genes and their relation with other soils
properties.
andre@kpu.ac.jpAndre F Cruz et al., J Ecosys Ecograph 2018, Volume 8
DOI: 10.4172/2157-7625-C3-039