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Journal of Ecosystem & Ecography | ISSN: 2157-7625 | Volume 8

July 11-12, 2018 | Toronto, Canada

International Conference on

Environmental Microbiology & Microbial Ecology

International Conference on

Ecology, Ecosystems & Conservation Biology

&

Nutritional and cultivational aspects of

Flammulina velutipes

(Curt. Fr.) Sing. under Punjab conditions

Gurpreet Kaur

Institute of Paramedical Health and Hygiene, India

F

lammulina velutipes

wasmaintained on Potato-Dextrose Agar (PDA) slants and used for physiological, nutritional and cultivational

studies. The culture showed white to cream coloured mycelium on seven different agar media with fine and strand hyphae on

Wheat extract, PDA and Malt extract medium while thin and silky character was seen on Czapeck's and Richard's medium. Among

the synthetic media, Richard's medium showed best growth.

F. velutipes

yielded maximum biomass on Wheat extract medium at

pH 6.5 when incubated at 25°C for 20 days. Nutritional studies using Richard's medium showed that glucose and mannitol were the

preferred C-sources while ammonium nitrate and glutamic acid were the preferred N-sources. Among the macro-elements, calcium

supplementation improved biomass yield while molybdenum was most effective among the trace elements tested. For cultivation,

different substrates were used including wheat straw, paddy straw, cotton waste, corncobs, sawdust and moong bean waste. Maximum

yield was obtained on wheat straw supplemented with 15% wheat bran (BE 14.28%). Minimum number of days for completion of

spawn run was of 19 days in case of cotton waste, corncobs, moong bean waste. Pinheads were observed in paddy straw and corncobs

but no mature fruit bodies developed. Nutritional analysis showed that the fruit bodies were rich in proteins containing 27.57% crude

protein out of which 81.65% was digestible, however soluble fraction of protein-4.5%, crude fat-4.25%, fibre-9.35% and ash-9.25% on

dry weight basis was found. The dried powder of this mushroom contained 0.031 mg/100g

β

-carotene and 2.26mg/100g vitamin E.

gurpreet.khurmi@yahoo.com

J Ecosys Ecograph 2018, Volume 8

DOI: 10.4172/2157-7625-C3-039