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Volume 7 Biosensors Journal
ISSN: 2090-4967 Electrochemistry 2018
June 11-12, 2018
June 11-12, 2018 | Rome, Italy
4th International Conference on Electrochemistry
Immunosensor for label-free PSA cancer detection on GQDs-AuNRs modified screen-printed
electrodes
Rajiv Prakash, Monika Srivastava and Narsingh R Nirala
Banaras Hindu University, India
Literature reveals that, in males, prostate cancer is ranked second as leading cause of death out of more than 200 different
cancer types. Prostate specific antigen (PSA) is a 33-kDa serine protease, which is largely bound to endogenous protease
inhibitors in human blood serum and its concentration in serum is used as indicator for prostate cancer. In healthy males the
PSA concentration level ranges from 0 to 4 ng ml-1 in the serum. There are several PSA detection methods available like enzyme-
linked immunosorbent assay (ELISA), radioimmunoassay, chemiluminescent immunoassay and SPR based immunosensors but
are complicated, costly and time consuming. There is urgent need for the development of low cost, user-friendly and quick
sensors for PSA. Recently, we have developed a simple and cost-effective biosensor for detection of PSA based on novel graphene
quantum dots decorated with gold nanorods (GQDs-AuNRs) and modified with PSA antibody coated over screen-printed
electrodes. The detection of PSA is demonstrated using three electrochemical techniques cyclic voltammetry (CV), differential
pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). A typical response for the PSA is shown in the
figure based on EIS technique. The modification of screen printed electrodes with novel hybrid of graphene quantum dots-gold
nanorods and simultaneous detection using three different techniques makes the sensor sensitive, reproducible and reliable. PSA
immunosensor shows 0.14 ng ml-1 limit of detection, which is capable of prediction of any disorder or chances of PSA cancer.
Recent Publications
1. Kashish, Sandeep Gupta, S K Dubey and Rajiv Prakash (2015) Genosensor based on nanostructured platinum modified
glassy carbon electrode for Listeria detection. Analytical Methods 7:2616-2622.
2. Madhu Tiwari, Ashish Kumar, Uma Shankar and Rajiv Prakash (2016) The nanocrystalline coordination polymer of
AMT–Ag for an effective detection of ciprofloxacin hydrochloride in pharmaceutical formulation and biological fluid.
Biosensors and Bioelectronics 85:529–535.
3. Madhu Tiwari, Ashish Kumar and Rajiv Prakash (2016) Nano-porous network of DMTD-Ag coordination polymer for
the ultra-trace detection of anticholinergic drug. Polymer 82:66-74.
4. Sandeep Gupta and Rajiv Prakash (2014) Photochemical assisted formation of silver nanoparticles by dithizone and its
application in amperometric sensing of cefotaxime. J. Mater. Chem. C 2:6859-6866.
5. Rajiv Prakash et al. (2017) Highly sensitive in vitro biosensor for enterotoxigenic Escherichia coli detection based on
ssDNA anchored on PtNPs-chitosan nanocomposite. Electroanalysis DOI: 10.1002/elan.201600169
Biography
Rajiv Prakash is a Professor and Coordinator of the School of Materials Science and Technology, Indian Institute of Technology, Banaras Hindu University, India. He has
served as Scientist at CSIR lab Lucknow, India for more than 7 years before joining Indian Institute of Technology. He has been recipients of Young Scientist (Council
of Science and Technology), Young Engineer Awards (INAE) of India and Materials Society Medal Award of India. His current research interests include synthesis of
morphology controlled organic conducting polymers, nanocomposites, fabrication and characterization of organic electronic devices and sensors/biosensors. He is having
more than 150 publications in international journals of repute and 17 patents in his credit. He is in Editorial Board of several National and International Journals. He is
Member of various national committees including DST-TIFAC for India Vision 2035 and MHRD IMPRINT program.
Rajiv Prakash et al., Biosens J 2018, Volume 7
DOI: 10.4172/2090-4967-C1-002