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Volume 6, Issue 8(Suppl) J Gastrointest Dig Syst 2016
ISSN:2161-069X JGDS, an open access journal
Page 31
Digestive Diseases 2016
December 08-09, 2016
conferenceseries.com
Digestive Diseases
December 08-09, 2016 Dubai, UAE
International Conference on
Calretinin expression in Hirschsprung disease – A potential marker of ganglion cells
Mishal Sikandar
Avicenna Medical College, Pakistan
Statement of Problem: Hirschsprung disease (HSCR) or congenital intestinal aganglionosis is a birth defect characterized by
complete absence of neuronal ganglion cells from a portion of the intestinal tract, mostly in a segment of rectum and variable length of
contiguous proximal, causing functional obstruction and colonic dilation proximal to affected segment. Routine diagnostic modalities
like Hematoxylin & Eosin (H&E) and Acetylcholine Esterase (AChE) staining as well as radiology-based clinical techniques have been
conventionally used for identification of aganglionosis and presence of hypertrophic nerve trunks in the affected segment as primary
indicators of HSCR. However, these conventional methods have their inherent deficiencies as H&E requires multiple trans-mural
biopsies and the interpretation of ganglion cells is often very difficult. Similarly, AChE requires fresh frozen section for which the
chances of technical error are very high and this facility is not commonly available in Pakistan. The number of misdiagnosed results
with potential overtreatment stands in need for reliable staining to prevent harm from unnecessary surgery and mortality. Recently,
Immunohistochemical markers are being increasingly used and evaluated in Pathology laboratories. No immunohistochemical
marker, either alone or in combination, has emerged from those researches that are as promising as calretinin. Hence, this study
was designed with an aim to observe the immunohistochemical expression of Calretinin as a marker for aganglionosis and to detect
ganglion cells in the affected areas for better and more accurate diagnosis of the disease.
Methodology: This study was conducted at University of Health Sciences Lahore, Pakistan from February to September, 2016.
Colonic Biopsy Specimens from 73 patients were collected mostly from Mayo Hospital, Lahore with established histopathologic
diagnosis of HSCR considered for the study. Age range was 0.1-120months. There were 48 (65.8%) cases who were ≤12 months old,
20 (27.4%) were 12.1-60 months old and 5 (6.8%) of the cases were 60.1-120 month old. The mean age of patients was 12.52±9.21
months. There were 52 (71.23%) male and 21 (28.77%) female patients. The male to female ratio in this study was 2.48:1. According
to sign and symptoms and clinical examination, 69 (94.5%) cases had mostly long standing constipation, 47(64.4%) cases had fever,
68(93.2%) cases had vomiting, 31(42.5%) cases had failure to thrive, 20(27.4%) cases had Enterocolitis and 63(86.3%) of the patients
had palpable abdominal masses. Methodology involved staining of fresh sections with H&E procedure for provisional histological
diagnosis. The biopsies were then processed for immunohistochemical staining with Calretinin and were observed for presence of
ganglion cells.
Findings: All the ganglion cells took brownish-black stain and were easily identified, which were not being identified on H&E.
Ganglion cells were present and absent in 42(57.53%) and 31(42.47%) respectively. The study revealed that the Calretinin
immunohistochemistry was very sensitive and specific for detecting ganglion cells.
Conclusion & Significance: It was concluded that Calretinin provides a very reliable and cost effective adjunctive test to be routinely
used with H&E in the evaluation of Rectal Section Biopsies (RSBs) for HSCR. The use of Calretinin may help the Pathologists in
making accurate and reliable diagnosis for HSCR and consequently eliminating the need for repeated biopsies and unnecessary
surgeries.
Biography
Mishal Sikandar is a Doctor and obtained MBBS from UHS, Pakistan in 2012. He worked as House Officer in Medicine, Cardiology and Obs & Gynae in 2013. He then
worked in Obstetrics and Gynecology for one year and started Post-graduation in Morbid Anatomy and Histopathology, from UHS, Lahore, Pakistan. His research interests
are cardiology, female genital tract pathologies, carcinoma breast, gastrointestinal pathologies, bone cancer and Hirschsprung's Disease. He has published articles in
Biomedica, an official publication of University of Health Sciences.
Mishal Sikandar, J Gastrointest Dig Syst 2016, 6:8(Suppl)