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Clinical Gastroenterology 2016
October 03-05, 2016
Volume 6, Issue 6(Suppl) J Gastrointest Dig Syst
ISSN:2161-069X JGDS, an open access journal
conferenceseries .com
October 03-05, 2016 Toronto, Canada
8th International Conference on
Clinical Gastroenterology & Hepatology
Kianosh Vahedi, J Gastrointest Dig Syst 2016, 6:6(Suppl)
Expanding and characterizations of colon cancer-derived stem cells
Kianosh Vahedi
Shahid Beheshti University of Medical Science, Iran
Background: Increasing and spreading out of cancer stem cells as spheroids were verified in our previous studies. But, capability
of primary tumor-derived stem cells to keep their unique properties in vitro is still doubtful. So, the goal of this study was to
segregate, expand and characterize the colon cancer-derived stem cells.
Materials & Methods: In the present work, colon cancer stem cells indicators including CD44 and EPCAM in spheroid and
maternal cells were analyzed by flow cytometry. The appearance levels of stemness genes in both spheroid and parental cells
were investigated using real-time PCR. Tumorigenic potential of spheroid cell was evaluated and used for the implantation of
tumor xenografts into nude mice.
Results: The earned data showed 75% of spheroids were CD44+/EpCAM+, while parental cells only expressed 18% of CD44/
EpCAM markers (p<0.01). In comparison with the parental cells, the expression levels of stemness genes, like Sox2 , Oct4,
Nanog, C-myc, and Klf4 were significantly increased in spheroid cells (p<0.05). Furthermore, as little as 1000 spheroid cells
were sufficient to obtain tumor growth in nude mice, while 1x106 of parental cells was needed to generate tumor.
Conclusion: Sphere formation test is a useful method to enhance cancer stem cells. Spheroid cells showed increasing
manifestation of stemness genes and tumorigenic activity in nude mice.
Biography
Kianosh Vahedi is a student of Oncology at the Shahid Beheshti University of Medical Science, Iran. He is pursuing his PhD from Shahid Beheshti University of
Medical Science, Iran. He has published more than 15 papers in reputed journals and has been serving as an Editorial Board Member of repute.