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Clinical Gastroenterology 2016
October 03-05, 2016
Volume 6, Issue 6(Suppl) J Gastrointest Dig Syst
ISSN:2161-069X JGDS, an open access journal
conferenceseries .com
October 03-05, 2016 Toronto, Canada
8th International Conference on
Clinical Gastroenterology & Hepatology
Novel in vitro three dimensional cultures of human intestinal cell lines to develop 3D model for
inflammatory bowel disease
Rasha Dosh, Nicola Jordan-Mahy, Christopher Sammon and Christine Le Maitre
Sheffield Hallam University, UK
In order to develop an in vitro 3D cell culture model which mimics the natural environment of the small intestine, pNIPAM-
Laponite hydrogel system was investigated. Human colonic adenocarcinoma cell lines: Caco-2 and HT29-MTX have been
widely used in in vitro 3D culture system as these cells have the ability to differentiate into enterocyte-like cells and mucus
producing goblet cells respectively; and exhibit the properties of intestinal epithelia. For these reasons each cell line and co-
cultures were investigated in suspension and layered cultures using the novel pNIPAM hydrogels, cultures were maintained
under static culture or dynamic culture for up to 8 weeks. Cell viability was assessed using Alamar Blue assay, and histological
stains: H&E, Alcian Blue-Periodic Acid Schiffs (PAS) were used to investigate cellular morphological and matrix production.
Scanning electron microscopy (SEM) was also used to assess the morphology of cells within the hydrogel. Both cell types
remained viable and those cultured in layered cultures under dynamic culture formed villus like structures and produced both
acidic and neutral mucins. SEM analysis showed the presence of cells within/on the surface of the hydrogel, where cells formed
circular clusters of cells forming mosaics with each cell having microvilli. We conclude that the pNIPAM-Laponite hydrogel
could provide a novel 3D intestinal in vitro model.
Biography
Rasha Dosh has completed her MSc from Al-Mustansiriyah University and worked as a Lecturer at University of Kufa College of Medicine, Iraq. She has published
4 papers in College of Medicine journals. She is currently a second year PhD student at Sheffield Hallam University, UK.
Rasha Dosh et al., J Gastrointest Dig Syst 2016, 6:6(Suppl)