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Volume 8, Issue 9 (Suppl) J Earth Sci Clim Change
ISSN: 2157-7617 JESCC, an open access journal Climate Congress 2017
October 16-17, 2017
October 16-17, 2017 Dubai, UAE
3rd World Congress on
Climate Change and Global Warming
Prevalence and characterization of methicillin-resistant Staphylococcus aureus isolates from normal
working places
Sayantan Chatterjee and Sangeeta Menon
Indian Academy Centre for Research & PG Studies, India
Statement of the Problem: Substantial amounts of pharmaceuticals are used in human and veterinary medicine. The inherent
biological activity of these non-regulated pollutants turns their occurrence in the aquatic systems into an environmental
concern and leads to the selection of antibiotic resistant bacteria in the environment. Therefore, emergence of antibiotic
resistant bacteria, such as Methicillin Resistant Staphylococcus aureus (MRSA), has become major hurdle in treatment of
various deadly diseases.
Methodology &Theoretical Orientation: The present study was aimed at determining the prevalence of MIRSA in restroom
and classroom door handles of the college. 12 locations were selected in the college campus and 21 samples from both the
restrooms and classroom handles were collected using sterile cotton swabs dipped in buffered peptone water and transported
to the lab. Isolation of S. aureus was carried out in mannitol salt agar and the isolates were identified by Gram staining and
biochemical tests. Antibiotic susceptibility of these isolates was done by disk diffusion method. Genomic DNA was isolated
and purified and is being studied further for coa and spa genes.
Findings: Four MRSA isolates were obtained and identified. Coagulase tests were found to be negative and their antibiotic
sensitivity revealed that the isolates were resistant to ampicillin, amoxycillin, cefoxitin and cefixime. Further sequencing work
is being carried out.
Conclusions: In this study, we have the presence of MRSA in different areas of a normal working place. The presence of
MRSA in the study emphasizes the need to formulate hygiene measures to prevent possible spread of MRSA and the other
transmissible pathogens to students and faculties in the college.
Biography
Sayantan Chatterjee is currently studying in Indian Academy Centre for Research & PG Studies in India. He has completed his Bachelor’s degree of Science in
Microbiology, Genetics and Biochemistry. His research interest lies in environmental microbiology, health, cancer biology, plant biotechnology, molecular biology
and immunology.
Sayantan Chatterjee et al., J Earth Sci Clim Change 2017, 8:9 (Suppl)
DOI: 10.4172/2157-7617-C1-033