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Volume 8, Issue 9 (Suppl)
J Earth Sci Clim Change
ISSN: 2157-7617 JESCC, an open access journal
Climate Congress 2017
October 16-17, 2017
October 16-17, 2017 Dubai, UAE
3
rd
World Congress on
Climate Change and Global Warming
Bio-surfactant production by naphthalene degrading bacteria from oil contaminated soil samples
Shah Jiten J and Sangeetha Menon
Indian Academy Centre for Research & PG Studies, India
Introduction & Aim:
The major environmental pollution of soil and water is due to hydrocarbon contamination resulting by
the petrochemical industrial activities. Polycyclic aromatic hydrocarbons (PAHs) such as naphthalene are hazardous class of
organic compounds produced as a result of pyrolysis of fossil fuels or other organic matter. Soil microorganisms have the ability
to utilize hydrocarbons as a carbon source. The present study was aimed at isolating bio-surfactant producing bacterial strains
capable of degrading naphthalene.
Methodology:
Fifteen (15) oil contaminated soil samples collected from four wheeler garages and petrol stations of North
Bangalore, Karnataka, India were enriched in Bushnell Haas (BH) medium with naphthalene as a sole carbon source for 7 days
followed by spread plate on BH Agar (BHA) medium. Naphthalene degrading colonies obtained on BHA were purified and
maintained in nutrient agar slants. The isolates were characterized up to genera by morphological and biochemical characters.
Bio-surfactant production was tested in the isolates by various screening methods such as drop collapse method, emulsification
activity, etc. Most efficient isolates were identified up to species by molecular characterization and analyzed for bio-surfactant
production using cheap carbon sources.
Results:
Fifteen naphthalene degraders were isolated from oil contaminated soil samples and were identified as members of
genera
Bacillus, Staphylococcus, Enterobacter, Stenotrophomonas
and
Klebsiella
. Two most efficient bio-surfactant producers
were identified as
Staphylococcus arlettae
and
Stenotrophomonas maltophilia
. Considerable amount of bio-surfactant production
was observed by these isolates in BH medium supplemented with cheap carbon sources.
Conclusion:
Naphthalene degrading isolates capable of bio-surfactant production could pave a way for effective bioremediation
of oil contaminated soil and water environments.
Biography
Shah Jiten J is currently working as a Research Fellow at Indian Academy Centre for Research & PG Studies in India after completing his Masters in Microbiology.
His research interest lies in the field of environmental microbiology, biotechnology, cancer biology, genetics and molecular biology.
jitenshah1193@gmail.comShah Jiten J et al., J Earth Sci Clim Change 2017, 8:9 (Suppl)
DOI: 10.4172/2157-7617-C1-033