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Volume 8, Issue 9 (Suppl) J Earth Sci Clim Change
ISSN: 2157-7617 JESCC, an open access journal Climate Congress 2017
October 16-17, 2017
October 16-17, 2017 Dubai, UAE
3rd World Congress on
Climate Change and Global Warming
Tobacco, a platform for efficient biofuel production: Pre-treatment to bioethanol production from
lignocellulosic biomass of tobacco
Cotek Temitayo, Kokila S, Mahuya De Ghosh, Prashanthi Karyala and Inla Sravani
Indian Academy Centre for Research & PG Studies, India
Statement of the Problem: The escalating industrial and domestic demands on non-renewable energy resources have led to
the rapid depletion of fossil fuels. This has resulted in the emergence of bioethanol derived from fermentation of food crops
such as maize and corn which has increased the prices of food commodities. Second generation bioethanol based on raw
materials rich in complex carbohydrates such as cellulose reduces the competition with the food industry. Tobacco is grown
in large fields all over the world and generates multiple harvests per year, thus producing large amounts of inexpensive green
biomass. The process to obtain second generation bioethanol involves four basic steps: pretreatment, enzymatic hydrolysis,
sugar fermentation and ethanol recovery.
Methodology & Theoretical Orientation: The dried tobacco leaves and stalk were pretreated with water, buffer (0.1 M Citrate
buffer) and dilute acids (H2SO4, HCl and HNO3 at 1%, 4% and 6%) at different temperatures (60oC, autoclave-121oC and 130 oC)
and microwave treatment (700 W, 2 min). The percentage of cellulose in the pre and post treated biomass was estimated by the
method of Updegraff. The pretreated biomass was subjected to enzymatic hydrolysis using cellulose from Trichoderma reesei (~700
U/g of substrate) and β-glucosidase (60 U/g of substrate). The total yield of glucose and ethanol produced for each pretreated
biomass was assayed by standard procedures.
Findings: A considerable loss of biomass was observed after pretreatment with dilute acids compared to pretreatment with
steam in water or citrate buffer. The highest glucose and ethanol yield was obtained in the pre-treated stalk with steam at
121oC in citrate buffer.
Conclusion & Significance: Results from the presented experimental work indicate that leaves and stalk of tobacco have a vast
potential for the production of sugars that eventually can be used for producing bio-ethanol. Despite declining cigarette sales
worldwide, the use of tobacco to produce bio-ethanol can be an alternative approach to save tobacco farmers. As tobacco is not
a food source it will not drive up food prices.
Biography
Cotek Temitayo has completed his Bachelor’s degree in Microbiology, Genetics and Biochemistry. He has done research projects on few topics such as isolation
and enumeration of endophytic fungus from medicinal plants, antibiotic resistance of pathogenic bacteria (Staphylococcus aureus) and population genetics study
on diabetes mellitus.
Cotek Temitayo et al., J Earth Sci Clim Change 2017, 8:9 (Suppl)
DOI: 10.4172/2157-7617-C1-033