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Volume 9
Journal of Obesity & Weight Loss Therapy
ISSN: 2165-7904
JOINT EVENT
Childhood Obesity 2019
Diabetes Conference 2019
March 18-19, 2019
&
3
rd
World Congress on
Diabetes and Obesity
12
th
International Conferences on
Childhood Obesity and Nutrition
March 18-19, 2019 | Rome, Italy
Concurrent prebiotic supplement reverses hyperinsulinemia induced by early-life pulsed antibiotic in rats
Klancic T
1
, Wong J
1
, Choo A C
1
, Nettleton J E
1
, Chleilat F
1
, Laforest-Lapointe I
1
and
Reimer R A
1,2
1
University of Calgary, Canada
2
Cumming School of Medicine, University of Calgary, Canada
Background:
Early life exposure to antibiotics increases risk of obesity. Prebiotics improve metabolic health and
reduce fat mass. Our aim was to examine if early postnatal prebiotic supplementation when co-administered with
antibiotic can reduce obesity risk in metabolically challenged offspring.
Methods:
10 week old female Sprague-Dawley rats (n=20) were mated and their pups were cross-fostered when 19
days old. Dams with their litters were randomized to: 1)control [C], 2)antibiotic [A] (azithromycin; dose 10mg/kg/
day), 3)prebiotic [P] (10% oligofructose (OFS) oral suspension/diet), 4)antibiotic+prebiotic [A+P] and 5)lean conrol
[LC]. The first pulse of antibiotics/prebiotics was administered before weaning from d19-21 of life through a feeding
dropper. Animals were weaned onto a high fat high sugar diet (HFS), with prebiotic groups (P and AP) containing
10% OFS in their diet. Prebiotic groups remained on the diet until the last pulse of antibiotics. The second and third
pulse of antibiotic were given d29-31 and d38-40, respectively. Body weight was assessed weekly, fecal samples were
collected repeatedly and tissues and blood were collected at sacrifice (wk7 and wk10). Insulin tolerance test (ITT)
was performed wk9 of life.
Results:
Males and females given antibiotics(A) had higher body weight than any other group; in females (A) higher
fasting glucose, insulin and leptin was detected after the third pulse of antibiotics (wk7) when compared to P and
AP group and ITT revealed insulin resistance compared to other groups. Similarly, males were insulin resistant
compared to P and AP groups, with higher fasting insulin levels. Calculation of homeostatic model assessment
of insulin resistance (HOMA-IR) confirmed insulin resistance in males and females. Longitudinal microbiota and
hypothalamic/hepatic gene expression analysis is ongoing.
Conclusion:
Therapeutic doses of antibiotic administered to rats mirrored the concentration commonly used for
human children for an acute infection. Antibiotics increased body weights, impaired insulin production and insulin
sensitivity, but the effects were reversed with prebiotic co-administration.
Biography
Klancic T has completed her BSc in Scotland, she pursued her MSc in Nutrition and Biomedicine in Germany. In 2015 she joined Dr. Reimer’s laboratory in
Calgary, where she is currently completing her PhD in Nutrition, Metabolism and Genetics. Her goal is to become an expert in the application of nutrition and
metabolism to obesity, and conduct research on novel methods of improving maternal and child metabolic health.
teja.klancic@ucalgary.caKlancic T et al., J Obes Weight Loss Ther 2019, Volume 9
DOI: 10.4172/2165-7904-C1-090