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Journal of Gastrointestinal & Digestive System | ISSN: 2161-069X | Volume: 8
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October 29-30, 2018 | San Francisco, USA
International Conference on
Gastrointestinal Cancer and Therapeutics
4th World Congress on
Digestive & Metabolic Diseases
26th Annual Congress on
Cancer Science and Targeted Therapies
Innovative immunotherapies to treat cancers
Chien-Fu Hung
Johns Hopkins University, USA
We report the development of anti-human mesothelin mRNA chimeric antigen receptor transfected peripheral blood
lymphocytes (CARMA-hMeso), demonstrating the manufacture and cryopreservation of multiple cell aliquots for
repeat administration from a single human leukapheresis. We show that CARMA-hMeso cells recognize and lyse tumor cells
in a mesothelin-specific manner. Expression of CAR was detectable over approximately 7 days in vitro with a progressive
decline of CAR expression that appears to correlate with in vitro cell expansion. In a murine ovarian cancer model, a single
intra-peritoneal (IP) injection of CARMA-hMeso resulted in the dose-dependent inhibition of tumor growth and improved
the survival of mice. Furthermore, repeat weekly IP administrations of the optimal CARMA-hMeso dose further prolonged
disease control and survival. No significant off-target toxicities were observed. These data support further investigation of
CARMA-hMeso as a potential treatment for ovarian cancer and other solid mesothelin-expressing cancers1. In additional,
we have successfully developed a strategy to specifically target a therapeutic chimeric protein to tumor loci, which elicits
potent tumor-targeted killing through antigen-specific CD8+ immune responses. This strategy may provide a platform for the
delivery various anti-cancer molecules to the tumor loci as well as for coating tumor cells to circumvent immune tolerance in
order to generate therapeutic antitumor effects2.
Biography
Chien-Fu Hung is an associate professor of pathology and oncology and a professor of gynecology and obstetrics at the Johns Hopkins University School of
Medicine. He is a member of the Johns Hopkins Kimmel Cancer Center. His research focuses on the prevention and treatment of cervical and ovarian cancers.
Chien-Fu Hung, J Gastrointest Dig Syst 2018, Volume 8
DOI: 10.4172/2161-069X-C8-085