23 / 26
Page 60
Notes:
conferenceseries
.com
Volume 2, Issue 3 (Suppl)
Breast Can Curr Res, an open access journal
ISSN: 2572-4118
Breast Cancer 2017
June 15-17, 2017
June 15-17, 2017 London, UK
5
th
World Congress on
Breast Cancer
Enhancement of radiosensitivity of human endothelial and breast cancer cells with melatonin by
regulating angiogenesis and genes involved in estrogen biosynthesis
Alicia González-González, Alicia González, Carolina Alonso-González, Carlos Martínez-Campa, Javier Menéndez-Menéndez
and
Samuel Cos
University of Cantabria, Spain
E
nhancing the radiosensitivity of cancer cells is one of the most important goals in clinical radiobiology. Melatonin
exerts oncostatic effects on breast cancer by reducing estrogen biosynthesis in human breast cancer cells, surrounding
fibroblasts and endothelial cells and by regulating cytokines that influence tumor microenvironment. This hormone also has
antiangiogenic activity in tumoral tissue. Vascular endothelial growth factor (VEGF) produced from tumor cells is essential for
the expansion of breast cancer. Thus, the aim of the present study was to investigate whether melatonin sensitizes endothelial
cells to radiotherapy by regulating angiogenesis and estrogen biosynthesis. To accomplish this we used cocultures of HUVEC
cells with MCF-7 cells. The expression of genes was analyzed by RT-PCR. Cell proliferation was measured by the MTT
method, the migration of HUVECs was measured by the wound healing assay and tubulogenesis studies were performed in a
tubulogenesis multiplate system in vitro. Only the presence of malignant epithelial cells in the cocultures altered proliferation,
the expression of genes involved in the local biosynthesis of estrogens and VEGF, in endothelial cells. In addition, ionizing
radiation decreased cell proliferation and VEGF expression, and melatonin pretreatment 1 mM led to a significantly greater
decrease. Furthermore, the migration of endothelial cells and the tube formation were reduced with the radiation andmelatonin
pretreatment resulted in a significantly higher reduction. Our results demonstrate that melatonin could exert a cooperative
enhancement of radiosensitivity associated with the modulation of angiogenesis and local estrogen biosynthesis.
Biography
Alicia González González is a PhD student from Cantabria University School of Medicine. Currently, she is involved in breast cancer research and melatonin,
specifically in the sensitizing effects of melatonin to chemotherapy and radiotherapy for its antiangiogenic and antiadipogenic actions. She has published 2 papers
and has presented her work in 5 congress.
agonzalez.bq@gmail.comAlicia González-González et al., Breast Can Curr Res 2017, 2:3(Suppl)
DOI: 10.4172/2572-4118-C1-005