biotechnology-2017-posters-accepted-abstracts

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Volume 7, Issue 5 (Suppl)

J Biotechnol Biomater

ISSN: 2155-952X JBTBM, an open access journal

Biotechnology 2017

November 13-14, 2017

November 13-14, 2017 Osaka, Japan

World Congress on

Biotechnology

Genome sequencing, assembly, annotation and analysis of methicillin-resistance of

Staphylococcus aureus

strain SO-1977 reveals genes responsible for antibiotic resistance

Sofia Bashir Mohamed Ali

National University Research Institute, Sudan

Background:

Staphylococcus aureus

is a ubiquitous bacterial pathogen and a leading cause ofmorbidity andmortalityworldwide.

The epidemiology of infections is influenced by rapid and widespread emergence of multidrug-resistant methicillin-resistant

aureus

(MRSA).

Staphylococcus aureus

(MRSA) is a major cause of nosocomial infection in Sudan. The relatively small genome

size and rapid evolution of antibiotic resistance genes in the species have been drawing an increasing attention in public health.

To extend our understanding of the species and use the genome data for comparative genomic studies, we sequenced the whole

genome of Methicillin-resistant of

Staphylococcus aureus

strain SO-1977 isolated from Sudan.

Methods:

Genomic DNA was sequenced using the Illumina MiSeq. The complete genome was annotated and the presence of

antimicrobial resistance genes was identified.

Result:

The draft genome of MRSA strain SO-1977 consisted of 2,827,644 bp with a G+C content of 32.8 %, 2,629 predicted

coding sequences (CDSs) and 55 RNAs. The final assembly contained 151 contigs of N50 contig length of 62,783 bp and the

largest contig assembled measured was 146,886 bp. Comparative studies of the MRSA strain SO-1977 and MRSA 252 through

RAST server showed a total of 20 were annotated to antibiotic resistance genes. Interestingly, one gene related to methicillin

resistance and four-genes related to Tetracycline resistance were found only in SO-1977 strain.

Conclusions:

This study is the first to report on the whole genome sequence of a Sudanese MRSA isolate. Antibiotic resistance genes

found in the genome indicate the presence of antibiotic resistance mechanism prior to the usage of antibiotics. The finding of this

study would help to understand the evolution of resistance mechanism and dissemination of the resistance genes of MRSA

J Biotechnol Biomater 2017, 7:5 (Suppl)

DOI: 10.4172/2155-952X-C1-083