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Volume 7, Issue 5 (Suppl)

J Biotechnol Biomater

ISSN: 2155-952X JBTBM, an open access journal

Biotechnology 2017

November 13-14, 2017

November 13-14, 2017 Osaka, Japan

World Congress on

Biotechnology

Effect of solvent system on the extraction of phenolic compounds and antioxidant capacity of

Gloriosa

superb

Amit Bahukhandi, Anjali Barola, I D Bhatt and R S Rawal

GB. Pant National Institute of Himalayan Environment and Sustainable Development, India

loriosa superba

Linn. (Family: Colchicaceae), commonly known as Malabar glory lily; Kalihari; Glory lily is grown in

semi-shade open areas. The species is distributed throughout temperate zone of India, Burma, Malaysia, SriLanka at

an altitude of 2100 m above sea level. In India, it is found in southern parts to the mid hill zones of Himachal Pradesh,

Jammu Kashmir, Uttar Pradesh and Uttarakhand. The species is brilliant wavy-edged yellow and red flowers. The plants have

reported richest sources of colchicine and gloriosine. Recently, colchicine is reported prime importance for its possible use in

cancer treatment. The species has been reported to use in the Indian and Chinese system of medicine for its analgesic, anti-

inflammatory, antimicrobial and antitumor properties. In addition, it is used in the treatment of snake bite, skin diseases, fever,

inflammation and respiratory disorders by local communities. The rhizome and its paste is used for the treatment of colic,

paralysis, chronic ulcer, bruises, sprains and considered useful in promoting labor and expulsion of placenta. The essential oil

of the species is used in cosmetic industries. Therefore, rhizome portion of

Gloriosa superba

was sampled and analyzed for

polyphenolic and antioxidant capacity in different solvent system and for harnessing maximum potential. Results revealed a

significant variation (p<0.05) in analyzed parameters among solvent systems. Total phenolic content ranged between 0.54-

1.35 mg GAE/g; flavonoid 0.66-185 mg QE/g; flavonol 0.33-1.03 mg QE/g and tannins 1.08-3.47 mg TAE/g dry weight and

maximum exhibited in methanolic solvent. Similarly, antioxidant activities were determined

in vitro

assays varied significantly

(ABTS 2.67-4.09; NO 2.26-4.05; DPPH 1.24-4.58 and OH 0.14-041 mM AAE/100 g dry weight). Among different solvent

types, methanolic extract was recorded best for harnessing maximum antioxidant potential; however, highest reducing

antioxidant power (0.54 mM AAE/100 g dry weight) was found in acetone. Total phenolic content showed significant (p<0.05)

positive relationship with flavonoid (r=0.905); tannin (r=0.914) and antioxidant activity (ABTS-r=0.967; NO-r=0.994;

p<0.01; OH-r=0.927; p<0.05, respectively). Likewise, flavonol showed strong correlation (p<0.01) with tannin (r=0.978) and

hydroxyl radical scavenging antioxidant activity (r=0.971). Tannin positively correlated (p<0.05) with antioxidant activity

(ABTS-r=0.892; NO-r=0.914; OH-r=0.971; p<0.01, respectively). The results of the present study are indicative of the fact that

the species possess polyphenolic content and antioxidant activity and therefore, can be a source of natural antioxidant. Solvents

with moderate polarity such as methanol and acetone showed higher polyphenolics and antioxidant activity, therefore, can be

utilized for harnessing maximum polyphenolics content.

J Biotechnol Biomater 2017, 7:5 (Suppl)

DOI: 10.4172/2155-952X-C1-083