38 / 41
Page 95
conferenceseries
.com
Volume 8
Journal of Biotechnology and Biomaterials
ISSN: 2155-952X
Biomaterials 2018
March 05-06, 2018
March 05-06, 2018 | Berlin, Germany
3
rd
Annual Conference and Expo on
Biomaterials
Assessment of Molecular Diffusion in Polyelectrolyte Multilayer Matrix
David Sustr
1,2
, Antonin Hlavacek
3
, Claus Duschl
2
and
Dmitry Volodkin
4
1
University of Potsdam, Germany
2
Fraunhofer Institute for Cell Therapy and Immunology, Germany
3
Institute of Analytical Chemistry - Czech Academy of Sciences, Czech Republic
4
Nottingham Trent University, UK
Assessment of molecular diffusion is of high importance in fields of drug delivery systems, biomaterial development, cell
biology, etc. Assessment and comprehensive analysis of the diffusivity provides a deeper understanding of the diffusion
phenomenon and heterogeneity of biomaterials. This insight eventually may lead to a rational control over the diffusivity.
Fluorescence recovery after photobleaching (FRAP) is commonly employed to probe molecular diffusion by analysis of the
recovery of fluorescence after photobleaching of fluorescently labelled molecules. Despite FRAP being a popular method,
it is hard to analyze multi-fractional molecular diffusion due to limited possibilities of approaches for analysis. Here we
present a novel simulation-optimization-based approach (S-approach) that significantly broadens possibilities of the analysis.
In the S-approach, possible fluorescence recovery scenarios are primarily simulated and afterwards compared with a real
measurement while optimizing parameters of a model until a sufficient match is achieved. This makes it possible to reveal
multi-fractional molecular diffusion. The proposed S-approach is compared with a conventional, yet advanced analytical
solution based approach (A-approach) which involves fitting an analytical solution of molecular diffusion to FRAP recovery
profiles. The S-approach is superior for multi-fractional analysis compared to the analytical one, however, diffusion of a single
population of molecules can be assessed by either of the approaches.
Figure 1:
Left: Distribution of diffusion coefficients of cytochrome c loaded into (HA/PLL)24 multilayers. Right:
Schematics depicting various interaction states of cytochrome c in polyelectrolyte multilayer and corresponding keywords.
Recent publications
1. Sustr D, Hlavacek A, Duschl C, Volodkin D (2018) Multi-Fractional Analysis of Molecular Diffusion in Polymer
Multilayers by FRAP: A New Simulation-Based Approach. The Journal of Physical chemistry B 122,1323-1333
2. Selin V, Ankner JF, Sukhishvili SA (2017) Nonlinear Layer-by-Layer Films: Effects of Chain Diffusivity on Film Structure
and Swelling. Macromolecules 50, 6192-6201.
David Sustr et al., J Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C1-088