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Volume 8
Journal of Biotechnology and Biomaterials
ISSN: 2155-952X
Biomaterials 2018
March 05-06, 2018
March 05-06, 2018 | Berlin, Germany
3
rd
Annual Conference and Expo on
Biomaterials
J Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C1-089
Hemicelluloses as reducing and dispersing agents for fabrication of noble metals nanoparticles
Fozia Iram
1
, Mohammad S Iqbal
2
, A Yasmeen1
and
B Khan
1
1
Lahore College for Women University, Pakistan
2
Forman Christian College, Pakistan
Statement of the Problem:
The hemicelluloses are hetropolymers of reducing sugars having aldehydic group in equilibrium
with the cyclic form; thus they can reduce noble metal ions to metallic particles and can disperse particles in their network. The
metal NPs thus prepared are suitable for biomedical applications.
Methodology & Theoretical Orientation:
Hemicelluloses are of great value for fabrication of metal nanoparticles (NPs) due
to their reducing and dispersion power, hydrophilicity, low cost, high thermal stability and biocompatibility. Metal NPs of
copper (Cu), silver (Ag) and gold (Au) were rapidly obtained after mixing the aqueous suspensions of hemicelluloses, isolated
from various plant species with precursor metal salts. Effect of various parameters such as amount of hemicellulose, pH and
temperature was optimized by response surface methodology using Design Expert®10.0.0 software.
Findings:
The reduction of metal ions was observed by change in colour: yellowish to purple/blue/ruby red in case of gold
or colourless to yellow/brown in case of silver, depending upon the temperature and pH, as monitored by recording the
characteristic surface plasmon resonance (SPR) spectra in the 300-800 nm range. The synthesized NPs were spherical in shape
and size of particles was determined by XRD, electron microscopy, dynamic light scattering techniques. The size was purely
dependent on pH, amount of the hemicellulose and temperature of the reaction-mixture. The synthesized gold NPs were
proved to be nontoxic as in cytotoxic tests.
Conclusion & Significance:
The size of gold NPs (>30) obtained by certain hemicelluloses fits in the range which can hardly
pass into the nucleus of cell, so they are suitable carriers for drug delivery. On the other hand NPs of silver and copper, showed
dose-dependent antimicrobial and cytotoxicity activities, so have potential to be used as anti-cancer and anti-infective agents.
Moreover, these types of biopolymers can be modified toward new functionalities with metals or biomolecules.
fozia_iram@hotmail.com