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Volume 8
Journal of Biotechnology and Biomaterials
ISSN: 2155-952X
Biomaterials 2018
March 05-06, 2018
March 05-06, 2018 | Berlin, Germany
3
rd
Annual Conference and Expo on
Biomaterials
Soft pulsed laser technologies for the transfer and processing of organic and biological/vivid materials
Ion N Mihailescu
1
, Carmen Ristoscu
1
and
Adriana Bigi
2
1
National Institute for Laser Plasma and Radiation Physics, Romania
2
University of Bologna, Bologna, Italy
B
asic principles of laser pulses interaction with solid and liquid materials using advanced pulsed laser technologies are
introduced and new recent results in synthesis of biomaterial layers are reviewed. Selection by combinatorial pulsed
laser deposition of silver-doped carbon structures with reliable physical-chemical characteristics and high efficiency against
microbial biofilms is presented. In-vitro biological assays were carried out using a large spectrum of bacterial and fungal
strains,
i.e. Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Enterococcus faecalis and Candida
albicans
. The biocompatibility of the films was evaluated on MG63 mammalian cells. The optimal combination with reasonable
physical-chemical properties, efficient protection against microbial colonization and beneficial effects on human cells was found
for silver-doped carbon films containing 2 to 7 at.% silver. These mixtures can be used to fabricate safe and efficient coatings
of metallic implants, with the goal to decrease the risk of implant associated biofilm infections which are difficult to treat and
often responsible for implant failure. In our opinion, these characteristics recommend the films with more than 2 and less than
7 at.% Ag concentration as the best compromise for the development of a new generation of smart coatings for orthopedic,
cardiology or dental implants. Combinatorial - matrix-assisted pulsed laser evaporation was applied to synthesize crystalline
gradient thin films with variable composition of Sr-substituted hydroxyapatite and zoledronate modified hydroxyapatite. The
inhibitory action of zoledronate on osteoclast viability and activity is more efficient than that of Sr, which however plays a
greater beneficial role on osteoblast proliferation and viability. The technique allows to modulate the composition of thin films
and hence the promotion of bone growth and the inhibition of bone resorption. Thin films prepared by pulsed laser techniques
are identical in chemical composition, structure, morphology, and most likely functionality resembling the base material, as
proved by physical-chemical characterization and in-vitro testing. Combinatorial methods open the possibility to combine
and immobilize two or more organic materials on a substrate in a well defined manner by laser evaporation under protection.
ion.mihailescu@inflpr.roJ Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C1-089