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Volume 7, Issue 2 (Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal Biomaterials 2017
March 27-28, 2017
2nd Annual Conference and Expo on
March 27-28, 2017 Madrid, Spain
Eloisa Gonzalez-Lavado et al., J Biotechnol Biomater 2017, 7:2 (Suppl)
Multi-walled carbon nanotubes (MWCNTs) as cytotoxic drug delivery systems in the treatment of cancer
Eloisa Gonzalez-Lavado1, Esperanza Padin-Gonzalez1, Nerea Iturrioz1, Tomas Torroba2 and Monica L Fanarraga1
1University of Cantabria, Spain
2Universidad de Burgos, Spain
Our laboratory has focused on the intrinsic anti-proliferative, anti- migratory and cytotoxic effects of carbon nanotubes (CNTs).
We have shown how MWCNTs interact with microtubules assembling biosynthetic polymers triggering serious biomechanical
cellular defects that lead to cancer cell death. These properties of CNTs produce antitumoral effects in solid melanoma tumors in
vivo. The huge surface area of CNTs maximizes their ability to interact with many biological components and different chemicals,
constituting their biocorona. Taking into account these surface properties, we aimed to increase these intrinsic antitumoral effects
of CNTs functionalizing these nanomaterials with a well-known anti-tumoral drug (5-fluoracil) in vitro in melanoma cells and in
vivo in solid malignant melanomas produced by allograft transplantation in murine recipients. We have double-coated CNTs with
an internal chemical layer surrounded by a second coat of proteins. The first layer carrying chemicals, either a dye (as a proof-of-
concept) or a drug (5-fluoracil) and the second being a serum protein coating layer, both assembling the biocorona. The protein
coating serves for (1) CNTs recognition by cellular receptors, (2) endocytosis, (3) protection of the chemical component attached to
the nanotube surface until protein degradation that takes place at the lysosome, and (4) the release of the transported drug during
the first 5-9 hours next to the internalization process. CNTs loaded with 5-fluoracil double coated with serum proteins display a
significantly enhanced antitumoral effect in vitro and in vivo in mice bearing solid melanoma tumors.
Biography
Eloisa Gonzalez-Lavado is a PhD student in the Nanomedicine Group of the University of Cantabria (Spain). I did a chemistry Bachelor’s degree at the University of
Extremadura (Spain).I have an european interuniversity master’s degree in theoretical chemistry and computational modelling. Currently I am working with carbon
nanotubes and their biomedical aplications, especially in Cancer, studying their biocompatibility, their capability as nanocarriers and their own antitumoral effect.