biomaterials-2017-posters-accepted-abstracts

24 / 36

Page 79
Notes:
conferenceseries.com
Volume 7, Issue 2 (Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal Biomaterials 2017
March 27-28, 2017
2nd Annual Conference and Expo on
March 27-28, 2017 Madrid, Spain
Rabadan-Ros Ruben et al., J Biotechnol Biomater 2017, 7:2 (Suppl)
Nurse’s A-phase material enhance adhesion, growth and differentiation of human bone marrow-derived
stromal mesenchymal stem cells
Rabadan-Ros Ruben1, Aznar-Cervantes Salvador2, Mazón Patricia3, Ros-Tarraga Patricia 1, De Aza Piedad N 3 and Meseguer-Olmo Luis1
1Universidad Católica San Antonio de Murcia, Spain
2Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario, Spain
3Miguel Hernández University, Spain
Silicon (Si) is a trace element that enhances bone formation and maturation in the body; thus apatite ceramics containing Si are
expected to increase the speed of bony regeneration. The mesenchymal stem cells from human bone marrow ( ahMSCs) are a great
promise for cell-based therapies by their ability to differentiate into osteoblast in certain microenvironments. The purpose of this
study was to evaluate the effect of a well-characterized Nurse’s A-phase (7CaO·P2O5·2SiO2) ceramic compared to a control (tissue
culture polystyrene-TCPS) on osteogenic differentiation of ahMSCs in vitro. Alizarin Red-S (AR-s) staining, alkaline phosphatase
(ALP) activity, and collagen I (COLI) were evaluated. Also, field emission scanning electron microscopy (FESEM) images were
acquired in order to visualize the morphology of the cells. The entire surface was colonized after 28 days of culture in growth medium
(GM). Osteoblastic differentiation markers were significantly enhanced in cells growing on Nurse’s A phase ceramic and cultured
with osteogenic medium (OM), and cells acquired polygonal shape typical from osteoblasts, probably due to the role of silica to
stimulate the differentiation of ahMSCs. Moreover, calcium nodules were formed under the influence of ceramic material. Therefore,
it is predicted that Nurse’s A-phase ceramic would present high biocompatibility and good osteoconductivity, being a good candidate
to be used as a biomaterial for bone tissue engineering.
Biography
Rubén Rabadán Ros is a Biologist at the University of Murcia (UMU). He completed his MSc degree in Molecular Biology and Biotechnology from the same
University. Currently, he is a PhD student in Biomedical Sciences at Universidad Católica San Antonio, Murcia (UCAM), developing scaffolds based on the C2S-
TCP phase diagram and their in vitro and in vivo study.