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Volume 7, Issue 2 (Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal Biomaterials 2017
March 27-28, 2017
2nd Annual Conference and Expo on
March 27-28, 2017 Madrid, Spain
Antonio Abel Lozano-Pérez et al., J Biotechnol Biomater 2017, 7:2 (Suppl)
Silk fibroin nanoparticles as an efficient carrier for quercetin
Antonio Abel Lozano-Pérez1, Hector Correa Rivero2, María delCarmen Pérez Hernández3, Ana Pagán1, Mercedes G Montalbán4, Gloria Víllora4 and José Luis
Cénis1
1Imida Murciano Institute for Agricultural and Food Research and Development, Spain
2Centro Nacional de Sanidad Agropecuaria, Cuba
3Instituto Nacional de Ciencias Agrícolas, Cuba
4University of Murcia, Spain
In the last decades, several researchers have associated a flavonoid-rich diet with an increase in average life in Mediterranean area
and a related reduction in the frequency of cardiovascular diseases. Up to date, multiple formulations with different encapsulation
methods and carriers for Q have been described in order to improve the stability and bioavailability of flavonoids. This work describes
how silk fibroin nanoparticles (SFNs) are capable of adsorbing and releasing quercetin and how their integrity is highly preserved
when is adsorbed onto the nanoparticles, as confirmed by antioxidant activity assays. Quercetin loading onto SFNs was optimized in
terms of quercetin/SFNs ratio (w/w), time of adsorption and solvent mixture. Quercetin-loaded silk fibroin nanoparticles (QSFNs)
were characterized using the dynamic light scattering technique to measure the diameter (Z-Average) and Z-potential (ζ ). The size
of loaded particles reached 171±1 nm (PdI=0.190) and were slightly bigger than the empty SFNs 139±1 nm (PdI=0.158), while the ζ
potential of QSFNs in water shifted toward positive values, from –27.3±0.4 mV in empty SFNs to –17.1±2.4 mV in QSFNs. Protein
corona formation onto SFNQs was lower when the loaded quercetin increased due to the shielding effect of the flavonoid around the
nanoparticles. The antioxidant activity against DPPH• showed that the Q loaded in QSFNs not only retains the antioxidant activity
but also has a synergistic scavenging activity due the intrinsic antioxidant activity of the silk fibroin. Drug loading content (DLC)
and Encapsulation Efficiency (EE) varied with the relation between Q and SFN in the loading solution reaching a maximum values
of EE=70% and DLC of 0.7%. The sustained release of Q was observed during the experiment both in phosphate buffer saline pH
7.4 and simulated intestinal fluid pH 6.8 with an overall cumulative release of 40% after 24h. SFNQs fluorescence can be detected
in a L929 cell. The results point to SFNs as promising candidate for Q loading, transport and delivery with potential applications in
nanomedicine, while retaining their nano-size and their antioxidant properties.
Biography
Antonio Abel Lozano-Pérez completed BSc degree in Biochemistry and Chemistry from University of Murcia, Spain and gained a PhD in Chemistry from University
of Murcia, Spain. In 2010, he gained a position as PhD researcher in the Biotechnology Department of the IMIDA (Murcia, Spain) to develop new applications of
the silk fibroin nanoparticles. He has his expertise in chemistry of the silk fibroin and in processing the silk to obtain nanoparticles for drug loading and delivery
useful for nanomedicine. He has developed these nanoparticles after years of experience in research and development, both in the University of Murcia and IMIDA
Institutions.