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Volume 8, Issue 5 (Suppl)
J Bioremediat Biodegrad, an open access journal
ISSN: 2155-6199
Biofuels Congress 2017
September 05-06, 2017
Page 40
conference
series
.com
September 05-06, 2017 | London, UK
Biofuels and Bioenergy
6
th
World Congress on
Nallusamy Sivakumar, J Bioremediat Biodegrad 2017, 8:5(Suppl)
DOI: 10.4172/2155-6199-C1-007
Production of ethanol from pretreated waste paper through separate enzymatic hydrolysis and
fermentation
W
aste paper is considered as one of the major components of municipal and industrial wastes and has the potential to be
used as an excellent alternative feedstock for ethanol production. In this study, the effect of various pretreatments on
efficient hydrolysis of waste office paper and newspaper into sugars and subsequent production of ethanol through fermentation
was studied. The shredded papers were soaked in deionized water (5 % w/v), milled and dried at 60°C for 24 h. The dry matter
was then milled again to remove most physical barriers of cellulose structure. The prepared waste papers were subjected to
various pretreatments using sulphuric acid, phosphoric acid, sodium hydroxide and hydrogen peroxide at a concentration of
0.1, 0.5 and 1% (v/v). Pretreatment with H
2
O
2
(0.5 % v/v) at 121°C for 30 min was considered as the most effective method as
it increased the available cellulose, produced a high sugar yield, high delignification and less inhibitors formation. The effect of
single (37 FPU/g solids) and two enzyme mixture (37 FPU + 25 CBU/g solids) was carried out using 2% (w/v) solid loadings.
The solid loadings experiment was done by loading 1-4% (w/v) solids with 37 FPU + 25 CBU/g solids of enzyme loadings. The
enzymatic hydrolysis of pretreated office paper and newspaper with 3% solid loadings resulted in the sugar yield of 23.48 and
13.12 g/L with hydrolysis efficiency of 91.8 and 79.6 %, respectively. Further, the hydrolysates of office paper and newspaper
were used as a substrate to produce ethanol through fermentation using
Saccharomyces cerevisiae
resulted about 11.15 and
6.65 g/L with the productivity of 0.32 and 0.28 (g ethanol/L/h), respectively. Thus, the improved yields achieved through the
pretreatment with 0.5% H
2
O
2
and subsequent ethanol production suggested that the wastepaper could be used as a potential
feedstock for bioethanol production.
Biography
Nallusamy Sivakumar has expertise in Microbial Fermentation. His areas of research interests are enzyme production, bioenergy, biofuel and bioprocessing. He is work-
ing on the possible utilization of different waste materials as alternative, cheap and renewable substrates for the production of microbial enzymes and other value added
products. He established the Bioprocess Lab in Sultan Qaboos University and supervising the team of students working in bioprocessing. He is also working on bioactive
compounds. In this, he is concentrating on the effect of different plant extracts and their essential oils on pathogenic bacteria and their possible mode of action.
apnsiva@squ.edu.omNallusamy Sivakumar
Sultan Qaboos University, Oman